STRUCTURAL BIOLOGY PROGRESS PRESENTATION PDB ID 4 DIU

STRUCTURAL BIOLOGY PROGRESS PRESENTATION PDB ID: 4 DIU Melissa Hoff

BASIL BASICS • What is it? • Who is involved? • Project Overview

CENTRAL DOGMA (FOR STRUCTURAL BIOLOGISTS) Amino Acid Sequence Structure Function

OVERVIEW OF METHODS BLAST Dali Py. MOL Pfam Database Moltimate

BLAST • What is it? • What information was gathered from BLAST?

• What is it? • What information was gathered from DALI? DALI Chain Z-score RMSD Length of Alignment Number of residues Sequences Identity Description 6 nkg-A 42. 4 0. 6 244 245 72 LIP_VUT 5, C 4 L 3 dkr-A 32. 4 1. 9 233 241 30 Esterase D 4 ke 6 -E 31. 6 1. 5 209 226 33 5 xks-F 30. 6 1. 7 214 253 31 6 qe 2 -A 23. 2 2. 6 208 257 21 Thermostable Monoacylglycerol Lipase Monoacylglycerol lipase 507 g 0 A 22. 2 2. 5 214 310 15 3 pf 8 -A 23. 8 1. 9 202 251 16 Alpha/beta hydrolase family protein Cinnamoyl esterase

PYMOL IMAGES

PFAM • What is it? • What information was gathered? • Hidden Markov Model • What’s next?

MOLTIMATE • What is it? • Catalytic triad • What does this mean?

Hydrolase (Pfam) EC class 3 (Moltimate) RESULTS Carboxylesterase or lipase (BLAST) Lipase (Dali)

WHERE TO NOW? COVID-19 Molecular Docking Communicat e with biochemistry partners Final Project!

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