STR STR automatic typing STR Allele PCR product

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第五章 STR自动分型 (STR automatic typing)

第五章 STR自动分型 (STR automatic typing)

国际上通用的法医STR分型 Allele PCR product

国际上通用的法医STR分型 Allele PCR product

琼脂糖凝胶电泳 (Agarose gel electrophoresis) 聚丙烯酰胺凝胶电泳 (Polyacrylamide gel electrophoresis)

琼脂糖凝胶电泳 (Agarose gel electrophoresis) 聚丙烯酰胺凝胶电泳 (Polyacrylamide gel electrophoresis)

Examples of DNA in the News 2004 Indian Ocean Tsunami 2001 “ 911”, New

Examples of DNA in the News 2004 Indian Ocean Tsunami 2001 “ 911”, New York 2008 Wenchuan Earthquake

PCR-STR DNA提取 (DNA extraction) 银染 PCR扩增 PCR (amplification) PAGE CE (Silver stain) 荧光染料 (fluorochrome)

PCR-STR DNA提取 (DNA extraction) 银染 PCR扩增 PCR (amplification) PAGE CE (Silver stain) 荧光染料 (fluorochrome)

High-Throughput STR Typing on the ABI 3100 (16 -capillary array) 256 data points in

High-Throughput STR Typing on the ABI 3100 (16 -capillary array) 256 data points in 45 minutes with STR 16 plex and 16 capillaries

第一节 荧光标记STR复合扩增 (Fluorescent tags STR composite amplification)

第一节 荧光标记STR复合扩增 (Fluorescent tags STR composite amplification)

常用的复合扩增方法有2种： 银染系统 (Silver stain) 荧光标记STR自动检测系统 (Fluorescent tags STR automatic detection system )

常用的复合扩增方法有2种： 银染系统 (Silver stain) 荧光标记STR自动检测系统 (Fluorescent tags STR automatic detection system )

Methods for Parallel Sample Processing Multiplex by Size Multiplex by Dye Color Blue Green

Methods for Parallel Sample Processing Multiplex by Size Multiplex by Dye Color Blue Green Yellow Internal sizing standard in red Combined Multiplex by Number of Capillaries

6 -FAM VIC NED PET

6 -FAM VIC NED PET

单基因座扩增的条件 预变性(Initial denaturation) 变性 (denaturation) 95˚C, 45 s 97℃, 5 min 35 60℃, 60

单基因座扩增的条件 预变性(Initial denaturation) 变性 (denaturation) 95˚C, 45 s 97℃, 5 min 35 60℃, 60 min 延伸(stretch) 72˚C, 45 s 退火(annealing) 59˚C, 45 s

ABI ID试剂盒的扩增条件 预变性 95℃, 11 min 变性 95˚C, 1 min 35 60℃, 60 min

ABI ID试剂盒的扩增条件 预变性 95℃, 11 min 变性 95˚C, 1 min 35 60℃, 60 min 延伸 72˚C, 1 min 退火 59˚C, 1 min

Amp. FℓSTR® Identifiler ®试剂盒

Amp. FℓSTR® Identifiler ®试剂盒

Kit component

Kit component

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