Step 1 Always carry with 2 hands Step

• Step #1: Always carry with 2 hands

• Step #2: Only use lens paper for cleaning

• Step #3: Do not force knobs

• Step #4: Always store covered

• Step #5: Keep objects clear of desk and cords

Eyepiece Body Tube Revolving Nosepiece Objective Lens Stage Clips Diaphragm Light Arm Stage Coarse Focus Fine Focus Base

COPY! STEP #1: Take a sheet of lens paper and gently wipe each objective lens. STEP #2: Prepare your slide (wet mount) or choose an already prepared slide. STEP #3: Make sure the microscope is on the lowest objective lens (scanning 4 X magnification) STEP #4: Turn the microscope ON. STEP #5: Make sure the diaphragm is open to the maximum setting. STEP #6: Place your slide onto the stage and secure your slide with the stage clips. STEP #7: Move the slide around until you see the specimen while you are looking through the eyepiece. STEP #8: Use the coarse adjustment knob to bring the specimen into general focus. You may change your objective lens to low power (10 X magnification) at this time. STEP #9: Once your specimen is in focus, change the objective lens to high power (40 X magnification). STEP #10: Use the fine adjustment knob to bring the specimen into sharp focus. STEP #11: Sketch the specimen in your notebook or on your lab paper. STEP #12: Remove the slide from the stage STEP #13: Wipe each objective with more clean lens paper. STEP #14: Turn off the microscope STEP #15: Unplug the power cord and wrap it around the base of the microscope. STEP #16: If there is a cover, place it on the microscope. STEP #17: Return the microscope to a clean cabinet

How do you calculate the total magnification of a specimen? COPY! Eyepiece lens has a magnification of 10 X. Objective Lenses ◦ ◦ Scanning Lens has a magnification of 4 X. Low Power lens has a magnification of 10 X. High Power lens has a magnification of 40 X. Oil Immersion lens has a magnification of 100 X. Total Magnification = eyepiece magnification x objective lens magnification

Total Magnification Chart COPY! Objective Lens Magnification Times (multiply) Eyepiece Magnification Total Magnification Scanning (4 X) X 10 X Low Power (10 X) X 10 X High Power (40 X) X 10 X 40 X 100 X 400 X Oil Immersion (100 X) X 1000 X

Microscope Field of View COPY! • The diameter of the field of an optical light microscope is the field number, which is the diameter of the field view in mm measured at the intermediate image plane. • The field number is typically listed on the microscope eyepiece. • The higher your magnification, the smaller the microscope field of view will be.

Elodea Slide 1. Drop water on slide 2. Get an elodea leaf from your teacher 3. Place leaf on drop of water 4. Cover with the slide cover

Elodea Cells at 40 x

Elodea cells at 100 X

Elodea at 400 x

Clear nucleus in elodea cell

Cheek Slide 1. Drop water on slide 2. Scrape inside of cheek with toothpick for 7 seconds 3. Rub toothpick on drop of water 4. Raise your hand wait for your teacher to dye your cells 5. Cover with the slide cover THROW AWAY THE SLIDE AND THE COVER ONCE YOU ARE FINISHED!

Cheek cells at 40 x

Cheek Cells at 400 x

Cytoplasmic Streaming http: //www. youtube. com/watch? v=8 edk 6 n. G Mw. Ms&NR=1&feature=fvwp