Stem Cell Research Center Imaging Core Instruments Start

Stem Cell Research Center Imaging Core Instruments Start Will you image or perform microdissections? Does your specimen have a heartbeat? No Dissect Yes Image Caliper Lumina I IVIS Vivarium B 640 Recharge Rate: $40/hr Is your sample alive in a dish or dead and fixed to a slide? Fluorescent dissection imaging 0. 75 x to 11. 25 x GFP & RFP filter cubes Fiber optic light source Color camera Scopes with Incubation System Features Benefits Applications Nikon Ti-E Inverted Widefield Room 1402 Basic research microscope 4 x, 10 x, 20 x, 40 x, 60 x (oil), & 100 x (oil) DAPI, FITC, TRITC, Tx. Red, & Cy 5 filter cubes Equipped with Perfect Focus System Many vessel holders Control focus drift Reduces background Fluorescence Brightfield Phase Nikon Ti-E Inverted Widefield Room 3402 Basic research microscope 4 x, 10 x, 20 LWDx, 40 x, & 60 x (oil) DAPI, FITC, Tx. Red, & Cy 5 filter cubes Equipped with Perfect Focus System Many vessel holders Control focus drift Reduces background Fluorescence Brightfield Phase Compact benchtop system (scope-in-a-box) 10 x & 40 x DAPI, FITC, & TRITC Long-Pass filter cubes Self-contained unit Controls room light Fluorescence Brightfield Phase Olympus FV 3000 Laser Scanning Inverted Confocal Room 1402 Recharge Rate: $25/hr Zeiss PALM Micro. Beam Inverted Widefield Room 2406 Recharge Rate: $24/hr Laser Micro-dissection Pressure Catapulting (LMPC) 5 x, 10 x, 20 x, & 40 x DAPI, GFP, Rhod, & Cy 5 filter cubes Nikon SMZ 1500 Dissection Room 1402 Scopes without Incubation Keyence BZX-810 Inverted Widefield Room 2301 Laser! Manually Live animal imaging Fluorescence & Luminescence Isofluorane for three animals at a time Olympus FSX 100 Inverted Widefield Room 3401 Will you do it manually or require more precision with a laser? Compact benchtop system (scope-in-a-box) 4 x, 10 x, 20 x LWD, 40 x, & 63 x (oil) DAPI, GFP, TRITC, Tx. Red, & Cy 5 filter cubes Equipped with Auto Focus Optical sectioning module Self-contained unit Controls room light Large area scanning Corrects focus drift High-powered fluorescent live cell imaging 2 x ma-PMTs & 2 x Ga. As. P-PMTs 1. 25 x, 10 x, 20 x, & 40 x (oil) 405 nm, 488 nm, 561 nm, & 640 nm laser lines FUW, FBN, & FGW (RBG) filter cubes Tokai-HIT stage top incubator Olympus Tru. Spectral detection Galvo/resonant hybrid scanner Fast acquisition speeds Large area scanning Corrects focus drift Zoom feature Deconvolution image processing Fluorescence Brightfield Phase Fluorescence DIC System Features Benefits Applications Olympus FV 10 i-LIV Inverted Confocal Room 2406 Compact benchtop system (scope-in-a-box) Laser Scanning 10 x & 60 x (water) 405 nm, 473 nm, 559 nm, & 635 nm lasers Self-contained unit Controls room light Confocal imaging of live cells Fluorescence Phase Olympus Viva. View Inverted Widefield Room 2406 Olympus FV 3000 Laser Scanning Inverted Confocal Room 1402 Recharge Rate: $25/hr Stable live cell imaging 10 x, 20 x, & 40 x DAPI, GFP, m. Orange, Tx. Red, Hc. Red, & Cy 5 filter cubes High-powered fluorescent live cell imaging 2 x ma-PMTs & 2 x Ga. As. P-PMTs 1. 25 x, 10 x, 20 x, & 40 x (oil) 405 nm, 488 nm, 561 nm, & 640 nm laser lines FUW, FBN, & FGW (RBG) filter cubes Tokai-HIT stage top incubator Olympus Tru. Spectral detection Stable incubation settings Galvo/resonant hybrid scanner Fast acquisition speeds Large area scanning Corrects focus drift Zoom feature Deconvolution image processing Contact Information _____ Allia Fawaz, M. S. Fawaz. A@uci. edu (949) 824 -0430 SCRC Core Microscope Imaging Manager Gross Hall Building Manager Fluorescence Brightfield DIC Fluorescence DIC

Stem Cell Research Center Imaging Core Instruments Microscope Imaging Systems Features Benefits Olympus FV 3000 Laser Scanning Inverted Confocal Room 1402 Recharge Rate: $25/hr High-powered fluorescent live cell imaging 2 x ma-PMTs & 2 x Ga. As. P-PMTs 1. 25 x, 10 x, 20 x, & 40 x (oil) 405 nm, 488 nm, 561 nm, & 640 nm laser lines FUW, FBN, & FGW (RBG) filter cubes Tokai-HIT stage top incubator Olympus Tru. Spectral detection Galvo/resonant hybrid scanner Fast acquisition speeds Large area scanning Corrects focus drift Zoom feature Deconvolution image processing Keyence BZX-810 Inverted Widefield Room 2301 Compact benchtop system (scope-in-a-box) 4 x, 10 x, 20 x LWD, 40 x, & 63 x (oil) DAPI, GFP, TRITC, Tx. Red, & Cy 5 filter cubes Equipped with Auto Focus Optical sectioning module Self-contained unit Controls room light Large area scanning Corrects focus drift Fluorescence Brightfield Phase Olympus FV 10 i-LIV Inverted Confocal Room 2406 Compact benchtop system (scope-in-a-box) Laser Scanning 10 x & 60 x (water) 405 nm, 473 nm, 559 nm, & 635 nm lasers Self-contained unit Controls room light Confocal imaging of live cells Fluorescence Phase Olympus Viva. View Inverted Widefield Room 2406 Stable live cell imaging 10 x, 20 x, & 40 x DAPI, GFP, m. Orange, Tx. Red, Hc. Red, & Cy 5 filter cubes Olympus FSX 100 Inverted Widefield Room 3401 Compact benchtop system (scope-in-a-box) 10 x & 40 x DAPI, FITC, & TRITC Long-Pass filter cubes Self-contained unit Controls room light Fluorescence Brightfield Phase Nikon Ti-E Inverted Widefield Room 1402*, 3402** Basic research microscope 4 x, 10 x, 20 x. LWD**, 40 x, 60 x (oil), & 100 x (oil)* DAPI, FITC, TRITC, Tx. Red, & Cy 5 filter cubes Equipped with Perfect Focus Many vessel holders Reduces background Control focus drift Fluorescence Brightfield Phase Stable incubation settings System Applications Fluorescence DIC Fluorescence Brightfield DIC Other Imaging Systems Caliper Lumina I IVIS Room: Vivarium B 640 Recharge Rate: $40/hr Zeiss PALM Micro. Beam Inverted Widefield Room 2406 Recharge Rate: $24/hr Nikon SMZ 1500 Dissection Room 1402 Features & Benefits Live animal imaging Fluorescence & Luminescence Isofluorane for three animals at a time Laser Micro-dissection Pressure Catapulting (LMPC) 5 x, 10 x, 20 x, & 40 x DAPI, GFP, Rhod, & Cy 5 filter cubes Fluorescent dissection imaging 0. 75 x to 11. 25 x GFP & RFP filter cubes Fiber optic light source Color camera Contact Information _____ Allia Fawaz, M. S. Fawaz. A@uci. edu (949) 824 -0430 SCRC Core Microscope Imaging Manager Gross Hall Building Manager