STAINING MICROSCOPIC IDENTIFICATION OF BACTERIA I STAINING A
STAINING & MICROSCOPIC IDENTIFICATION OF BACTERIA
I. STAINING • A. GRAM POSITIVE – BLUE/DARK PURPLE • B. COUNTER STAIN - PINK
GRAM STAIN PROCESS • HTTPS: //WWW. YOUTUBE. COM/WATCH? V=SXA 46 K X FIOY
II. CELL SHAPE • A. Round = coccus (cocci) • B. Rod shaped = bacillus (bacilli) • C. cork screw= spirillum (spirilla) • D. comma = vibro • E. thin flexible spiral = spirochete
III. CELL ARRANGEMENT • A. Pairs = diplo • B. groups of 4 = tetra • C. chains = strepto • D. clumps = staphlyo
IV. 2 PART SCIENTIFIC NAME – ITALICIZED OR UNDERLINED • A. Genus name • - first • - capitalized • - may be abbreviated by first letter • - multiple species share a genus • B. Species name • - second • - not capitalized • - only one species has that genus & species name
B.
• Purple so…. • Gram positive • Rod shape so… • bacillus (bacilli) • Chains so… • streptobacilli
C.
• Pink so. . • gram negative • Rod shape so. . • bacillus (bacilli) • Not chain or pairs
A.
• Purple so. . • gram positive • Spherical shape so. . • coccus(cocci) • Clumps so … staphylo
D
• Pink so. . • gram negative • Corkscrew shape so. . • spirillum (spirilli) • Not chain or pairs
SPECIES CHARACTERISTICS: Species Gram +- Bacillus megaterium + Excherichia coli - Micrococcus luteus + Rhodospirillum rubrum - shape bacillus coccus spirillum arrangement Colony form Colony elevation Colony margin color convex Entire/ undulate white strepto Circular /irregular Slightly raised Entire cream single Round/ small convex Etire yellow staphylo Circular/ small convex Entire red sigle Circular/ small
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