SRC Protocol Clarification Acronyms BSL Bio Safety Level

SRC Protocol Clarification

Acronyms • BSL – Bio Safety Level • FTQ – Failure to Qualify

Known source to culture 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Known BSL 1 source to subculture 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Unknown source to culture 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Unknown source to standard plate to subculture 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Unknown source to selective media to subculture 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Unknown source to culture to slide 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

BSL 2 source to standard plate 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

BSL 2 source to standard plate to subculture 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Known BSL 1 source to plate to subculture, source provided to student 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Known BSL 2 source to plate subculture, source provided to student 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Lyophilized BSL 1 source to standard plate 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Lyophilized BSL 1 source to standard plate to subculture 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Lyophilized BSL 1 source to hydrating broth to nutrient cuvette, sealed and incubated. 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Bread molds 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Bread really molds 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Slime mold to filter paper and feed 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Slime mold to plate and not opened to feed 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Slime mold to plate and re-open to feed 1. BSL-1 2. BSL-2 3. No BSL classification

Okay for Juniors? 1. Yes 2. No

Ballistics project, certified officer supervises 1. FTQ 2. No FTQ

Ballistics project, dad supervises 1. FTQ 2. No FTQ

Archery project, dad supervises 1. FTQ 2. No FTQ

Archery project, certified expert supervises 1. FTQ 2. No FTQ

Lasers

Student collects ocean (or pond) water. Student wants to compare different fertilizers' effects on plankton growth. They set up containers at home, add fertilizer, organic fertilizer and manure to the containers. 1. BSL-1 2. BSL-2 3. No BSL classification Would it make a difference if manure was not used?

A student surveys Alzheimer's patients at a day care facility. They have all the proper paperwork, and have a qualified scientist who has a masters degree in library science, but is a technician in an Alzheimer's research unit at a research hospital. 1. FTQ 2. No FTQ What if the qualified scientist had been appropriate? (I. E. had came from background in Alzheimer’s care)

A student obtains oil-eating bacteria and grows them in petri dishes with different types of oil to look at efficacy of the bacteria on different weights of oil. Student does not open the petri dishes. 1. BSL-1 2. BSL-2 3. No BSL classification PHBA--must be done in a BSL-1 lab. Oil-eating bacteria are only exempt from being considered PHBA projects if not cultured

Student wants to study the effectiveness of different products at repelling deer. His back yard is adjacent to a conservation area, so he's going to do this at home. 1. FTQ 2. No FTQ • Vertebrate project--requires vertebrate forms. • Student will have to find some way to report mortality/assure no more than 15% weight loss to the animals • Essentially they will not be able to do this, especially if they are using some home-made products in their treatments.

Someone From SRC