Specii din genul Actinobacillus si Haemophilus cu semnificatie

Specii din genul Actinobacillus si Haemophilus cu semnificatie patologica pentru porc Dr. COSTIN STOICA www. tgw 1916. net REGNVM PROKARYOTAE

FAMILIA PASTEURELLACEAE GENURI: Ø PASTEURELLA Ø ACTINOBACILLUS Ø HAEMOPHILUS REGNVM PROKARYOTAE

Dendrograma privind relatiile filogenetice ale unor reprezentanti ai familiei Pasteurellaceae pe baza similaritatii secventei ARNr 16 S A. actinomycetemcomitans H. aphrophilus H. segnis H. haemolyticus H. influenzae H. aegyptius A. equuli A. suis A. ureae A. pleuropneumoniae A. lignieresii A. minor H. minor group A. porcinus A. indolicus H. parasuis (NCTC 4557) H. parasuis (strain Nagasaki) Pasteurella species A H. taxon C P. gallinarum H. paragallinarum

GENUL ACTINOBACILLUS l A. actinomycetemcomitans lignieresii equuli seminis capsulatus pleuropneumoniae minor porcinus indolicus suis ureae rossii muris hominis ( Bergey’s manual of Sistematic Bacteriology, 1994; Moller et al, 1996) REGNVM PROKARYOTAE

GENUL ACTINOBACILLUS l A. actinomycetemcomitans lignieresii equuli seminis capsulatus pleuropneumoniae minor porcinus indolicus suis ureae rossii muris hominis ( Bergey’s manual of Sistematic Bacteriology, 1994; Moller et al, 1996)

Specii din genul Actinobacillus cu importanta pentru porc l. A. pleuropneumoniae minor porcinus indolicus suis REGNVM PROKARYOTAE

Toxinele sintetizate de fiecare serotip de A. pleuropneumoniae REGNVM PROKARYOTAE

Actinobacillus suis – coloratie Gram

Caractere biochimice diferentiale intre reprezentanti ai genului Actinobacillus + : >90% tulpini pozitive, - : > 90% tulpini negative, d : 11 - 89% tulpini pozitive (Moler et al. , 1996)

GENUL HAEMOPHILUS l H. parasuis taxon C taxon D taxon E taxon F ‘minor group’ aegyptius influenzae aphrophilus haemolyticus segnis paragallinarum REGNVM PROKARYOTAE

GENUL HAEMOPHILUS l H. parasuis l H. taxon C l H. taxon D l H. taxon E l H. taxon F l H. ‘minor group’ l H. aegyptius l H. influenzae l H. aphrophilus l H. haemolyticus l H. segnis l H. paragallinarum

Specii din genul Haemophilus cu importanta pentru porc l H. parasuis l H. taxon C l H. taxon D l H. taxon E l H. taxon D l H. minor group REGNVM PROKARYOTAE

Haemophilus parasuis – coloratie Gram

Caractere biochimice diferentiale intre reprezentanti ai genului Haemophilus Caractere biochimice H. minor group H. parasuis Taxon C Taxon D Taxon E Taxon F Necesar factor V 100 100 60 100 Necesar factor X 0 0 0 Indol 0 0 0 100 Ureaza 100 0 0 Oxidaza 13 100 0 0 100 galactozidaza 100 0 100 80 100 fucosidaza 0 96 0 68 80 46 glucozidaza 70 4 100 64 20 89 Neuraminidaza 100 100 91 60 100 Red. nitratilor 100 100 96 30 100

Caractere biochimice diferentiale intre reprezentanti ai genului Haemophilus (continuare) Caractere biochimice H. minor group H. parasuis Taxon C Taxon D Taxon E Taxon F Arabinoza 0 0 100 86 0 0 Lactoza 91 0 0 96 0 32 Maltoza 100 100 20 100 Melibioza 78 0 0 96 0 18 Sucroza 100 100 10 100 Trehaloza 78 0 0 55 0 79 Rafinoza 100 0 93 Manitol 0 0 0 100 0 0 Sorbitol 0 0 0 100 0 0 Inozitol 0 4 100 77 0 0

BIBLIOGRAFIE: Andrew N. Rycroft, Lisa H. Garside, " Actinobacillus Species and their Role in Animal Disease", The Veterinary Journal , 2000, 159, 18 -36 Cristian Moller et al. , ‘V Factor Dependent Members of the Family Pasteurellaceae in the Porcine Upper Respiratory Tract’, Journal of Clinical Microbiology, Dec 1990, p. 2711 -2716 Durda Slavic et al. " Prevalence of O 1/K 1 and O 2/K 3 Reactive A. suis in Healthy and Diseased Swine", Journal of Clinical Microbiology, Oct 2000, p. 3759 -3762 Durda Slavic et al. "Comparative pathogenicity of different Actinobacillus suis O/K serotypes", The Canadian Journal of Veterinary Research, 81 -86 J. Van Ostaaijen et al. , " A. suis Strains Isolated from Healthy and Diseased Swine Are Clonal and Carry apx. ICABDvar suis and apx. IICAvar suis Toxin Genes", Journal of Clinical Bacteriology John G. Holt et al. , “Bergey’s Manual of Determinative Bacteriology 9 th Edition, 1994 Moller K. et al. , Oct. 1996, "A. minor sp. nov. , A. porcinus sp. nov. , and A. indolicus sp. nov. , Three New V Factor-Dependent Species from the Respiratory Tract of Pigs", International Journal os Systematic Bacteriology , Oct. 1996, 1131 -1137 Radu Moga M@nzat et al. , “Boli infecioase ale animalelor – bacterioze”, p. 157 -188, Ed. Brumar 2001 Vicki J. Rapp-Gabrielson et al. , "Prevalence of Haemophilus parasuis serovars among isolates from swine", Am. J. Vet. Res. Vol 53, No 5, 1992

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