Southern blotting Northern blotting Western blotting thermal cycler
Southern blotting
Northern blotting
Western blotting
증폭장치 (thermal cycler)
제자리부합법 1. Fluorescence in situhybridization (FISH)
Chromosome translocation (염색체전좌)
Array comparative genomic hybridization (a. CGH; 비교유전체부합법)
c. DNA microarray
1. Southern, Northern Blot • • • Distribute the oligonucleotide probes over a gel Radioactively labeled oligonucleotide one gene, one messenger 2. DNA array • Oligonucleotide probes are immobilized on a surface at micrometer distance • Sample is usually labeled with a fluorescent dye • many genes, many messenger <응용 분야> 유전자 발굴(gene discovery), 암 분류(tumor classification), 유전자 발현 형태에 따 른 질병 위험도 평가(risk assesment) 질병 예후 진단(prognosis prediction), 질병진단(disease diagnosis), 신약 개발(drug discovery). 독성학연구(toxicological research) 식품 안전성 평가, 유전병 연구 등
Epstein Barr Virus CISH
In situ-PCR In situ RT-PCR
세포자멸사(Apoptosis)
2. 1. 1. 4. 3.
괴사(Necrosis)
DNA 단편화 (DNA fragmentation)
CAD-mediated apoptotic DNA fragmentation
세포자멸사의 검출-TUNEL법 Td. T-mediated d. UTP Nick End Labelling Td. T • Terminal deoxynucleotidyl transferase • Template independent DNA polymerase • ss DNA or ds DNA의 3’-OH 말단에 deoxynucleotide 첨가
세포자멸사의 검출-DNA ladder법 • DNA fragmentation
세포자멸사의 검출-Annexin V 염색 • PS (phosphatidylserine)
자가포식(Autophagy)
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