Some properties of Cupressus sempervirens essential oils Hayfa

  • Slides: 1
Download presentation
Some properties of Cupressus sempervirens essential oils Hayfa Arguia, c, *, Salih Can Suner

Some properties of Cupressus sempervirens essential oils Hayfa Arguia, c, *, Salih Can Suner b, e, Oumayma Ben Youchret-Zalleza, Çağdaş Deniz Perizd, Gülen Türkerf, Seyhan Ulusoyd, Mossadok Ben-Attiac, Ayhan Orale, Yalçın Coşkunh and Hechmi Saida a Materials chemistry laboratory, Department of Chemistry, Faculty of Sciences of Bizerte, University of Carthage, Tunisia. b Department of Chemistry and Chemical Processing, Lapseki Vocational School, Canakkale Onsekiz Mart University, Canakkale, Turkey. c Biomonitoring of the Environment Laboratory (LR 01 / ES 14), Faculty of Sciences of Bizerte, University of Carthage, Tunisia. d Department of Biology, Faculty of Science, Suleyman Demirel University, Isparta, Turkey. e Department of Chemistry, Faculty of Science and Arts, Canakkale Onsekiz Mart University, Canakkale, Turkey. f Çanakkale Onsekiz Mart University Application in Science and Technology and Research Laboratory, Canakkale, Turkey. g Çanakkale Onsekiz Mart University Department of Food Technology, Faculty of Canakkale Applied Science, Canakkale, Turkey. h Department of Plant Production, Lapseki Vocational School, Canakkale Onsekiz Mart University Canakkale, Turkey. Abstract: The aim of this study is to investigate some properties of Cupressus sempervirens essential oil (EO). These oils were distilled by the method of steam distillation. The compounds of the EO were identified by GC-MS. The antioxidant activities of the EOs of this species were investigated. Gram negative and gram positive bacterial strains were used to assess the antibacterial properties. It was found that the C. sempervirens EO has notable antibacterial activity against S. aureus and E. coli. In the results obtained from the insecticidal effect tests, C. sempervirens EO has a toxic contact effect and manifest a repulsive effect against adult Sitophilus granarius. These latter are optimized at a dose of 0. 16 µL cm-2. It was concluded that C. sempervirens EO can use as antibacterial agent against S. aureus and E. coli, and insecticide against stored grain product' pest S. granarius. C. sempervirens is the only species of Cupressus existing in Tunisia, It was involved in traditional medicine and its fruit is known for the treatment of diabetes and as an antiseptic. The EO of C. sempervirens has antimicrobial properties and so can be used as a natural antimicrobial agent for human and infectious diseases as well as for food preservation. In addition, the development of natural antimicrobial agents helps to reduce the negative effects (environmental pollution, resistance) of synthetic chemicals and drugs (1). According to several studies, many secondary plant metabolites have insecticidal properties (2) and some chemical constituents of EOs (3). It is known that plants are used as to kill or repel insects (2). The EOs have harmful, anti-agent, and anti-feeding effects on stored product insect pests (4 -6). Eucalyptus oil and cypress oil have high toxic effects against insect species like Sitophilus zeamais (LD 50=0. 36 µL cm-2). Studies on aromatic plants and their products have increased due to the increased interest in the need of natural products in recent years. It is also a fact that such natural products will generate income for many people by transforming them into commercial products. The present study aims to assess the antioxidant, the antibacterial, and the insecticidal activities of EOs from the aerial part of C. sempervirens against four strains and S. granarius, and to find correlations with their chemical compositions. Antioxidant properties Chemical composition The EO of this part of the plant has been identified with CG-MS. The main constituents are Samples α-pinene (39. 08 %), followed by delta-3 -carene (19. 96 %), α-Terpinenylacetate (5. 94 %), and d. L-Limonene (5. 56 %). The richness of the C. sempervirens EO in α-pinene has been DPPH Beta-carotene bleaching test IC 50 (µg m. L-1) Inhibition (%) RAA 60 min RAA 120 min C. sempervirens 10. 031 20. 464 0, 65 0, 56 BHT 1. 41 98 1, 254 1, 245 Negatif control a - - 0, 636 0, 553 confirmed by several authors in different countries: Algeria (44. 9%) ( 7), Italy (31%) (8). Antibacterial activity The antibacterial activity of C. sempervirens EOs against S. aureus, E. faecalis, E. coli and P. a Negatif control. Linoleic acid and β-Carotene emulsion aeruginosa was tested using the agar well diffusion method. The evaluation of the According to these results, the evaluation of the antioxidant activity in vitro by the DPPH test antibacterial activity of the EO of C. sempervirens exhibit remarkable bactericidal activity shows that the EO of C. sempervirens are characterized by a low antioxidant power (IC 50 = against S. aureus ATCC 25923 and E. coli ATCC 25922. On the other hand, none of the EO 10. 031 µg m. L-1) (Table 1). However, in the β-carotene bleaching test, it reveals a higher inhibited E. faecalis and P. aeruginosa. This high activity is due to their chemical composition. relative antioxidant activity (RAA), which is almost equivalent to 0, 65 μg m. L-1 after 60 min. Indeed, the EO of C. sempervirens, which has been shown to be active against bacterial This value slightly decreased after 120 min. strains (S. aureus ATCC 25923 and E. coli ATCC 25922), is rich in α-pinene (37. 568%). Thus, Conclusion we can conclude that α-pinene has antibacterial power. Contact toxicity test results C. sempervirens EO exhibited low antioxidant activity (IC 50= 10. 031 µg m. L-1) in the DPPH free radical scavenging test comparable to that of the reference antioxidants used. In addition, the beta-carotene discoloration inhibition test demonstrated good antioxidant activity of this oil. The study highlighted the screening of the antibacterial activity of the EO of C. sempervirens against S. aureus and E. coli strains, according to the method of diffusion in an agar medium (disc method). The inhibition diameters varied depending on the bacterial strain tested. Another highlight of the study is the C. sempervirens EO had contact toxic effects and repulsive effects against S. granarius adults. The final conclusion of the study reveals that C. sempervirens EO is promising natural component to combat some bacterial strains and stored cereal seed insects. References (1) Mazari K, Bendimerad N, Bekhechi C, Fernandez X. Chemical composition and antimicrobial activity of essential oils isolated from Algerian Juniperus phoenicea L. and Cupressus sempervirens L. J Med Plants Res. 2010; 4: 959 -964. (2) Broussalis AM, Ferraro GE, Martino VS, Pinzón R, Coussio JD, Alvarez JC. Argentine plants as potential source of insecticidal compounds. J. Ethnopharmacol. 1999; 67: 219 -223. (3) SIMÕES Cd. O, SPITZER V. Óleos voláteis. Farmacognosia: da planta ao medicamento. 2004; 5: 467 -495. (4) Regnault-Roger C. The potential of botanical essential oils for insect pest control. Integrated Pest Management Reviews. 1997; 2: 25 -34. (5) Isman MB. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annu Rev Entomol. 2006; 51: 45 -66. (6) Hafez M, Dimetry NZ, Abbass M. Insecticidal and biological efficacy of two vegetable oils against Callosobruchus maculatus (Fabricius)(Coleoptera: Bruchidae) under laboratory conditions. Archives of Phytopathology and Plant Protection. 2014; 47: 1942 -1955. (7) Chanegriha N, Baâliouamer A, Meklati B-Y, Favre-Bonvin J, Alamercery S. Chemical composition of Algerian cypress essential oil. J. Essent. Oil Res. 1993; 5: 671 -674. (8) Romeo FV, De Luca S, Piscopo A, Poiana M. Antimicrobial effect of some essential oils. J. Essent. Oil Res. 2008; 20: 373 -379. th