Slide spot pingroup subarray gene Affymetrix 11 25

ДНК Микрочип Slide spot pingroup subarray gene

Affymetrix ДНК чип Олигонуклеотиди: • 11 -25 мери (short) • 50 -70 мери (long) http: //en. wikipedia. org/wiki/Image: Affymetrix-microarray. jpg Nelzo Ereful Crop Bioinformatics

РНК екстрякция Images by Microarray Mediabook. http: //gcat. davidson. edu/Pirelli/index. htm

к. ДНК синтез и багрила Cy 3 (green) Control/ well-watered Cy 5 (red) Experimental/ stressed Images by Microarray Mediabook. http: //gcat. davidson. edu/Pirelli/index. htm

РНК деградация Nelzo Ereful Crop Bioinformatics Images by Microarray Mediabook. http: //gcat. davidson. edu/Pirelli/index. htm

к. ДНК библиотека IR 64 wellwatered panicles IR 64 stress extract Total RNA c. DNA library PCR 15 cycles genes (Katherisan, 2005).

ДНК микрочип анализ Хипотетичен геном Images by Microarray Mediabook. http: //gcat. davidson. edu/Pirelli/index. htm

Хибридизация Quackenbush, 2006

Сканиране на имиджа Microarray Mediabook on scanning Images by Dr. John Bennett, IRRI

Обработка на картината Images by Dr. John Bennett, IRRI

Експресионно съотношение ∆ Генна експресия R G 600 1200 T= 0. 07 Експеримент G Контрола 5600 11600 13000 15500 18000 17500 16500 13500 0. 03 R 0. 4 10900 6500 1. 0 2500 800 6. 2 22. 5 0/0

Фолд/Пъти изменение R G Рецепрочна ст. 600 1200 5600 11600 13000 15500 18000 17500 16500 13500 0. 03 0. 07 0. 4 33. 3 14. 3 2. 5 Fold Repression 10900 6500 1. 0 2500 800 6. 2 22. 5 Fold Induction

Асиметрично разпределение 0. 03 0. 07 0. 4 1. 0 2. 0 6. 2 22. 5 Image by Microarray Mediabook. http: //gcat. davidson. edu/Pirelli/index. htm

Нормализация/Трансформация • log 2 transform 0. 03 0. 07 -5. 1 -3. 8 Repression 0. 4 -1. 3 1. 0 2. 0 6. 2 22. 5 0 1. 0 2. 6 4. 5 Induction Image by Microarray Mediabook. http: //gcat. davidson. edu/Pirelli/index. htm