Sequencing Batch Biological Reactor SBBR l SBR MBR

Sequencing Batch Biological Reactor (SBBR)連續批分式生物反應槽 l SBR＋ MBR (membrane) l 處理ABS 廢水: 有90% COD 及87. 4% TN去除率

Sequence of oligonucleotide probes used in this study Probes were labeled with FITC at 5’ end

Eub probe Alc probe Cory probe Aci probe Fig. Hybridization results of Eub 338 to mixture of target cells (upper), and Alc probe was used in the bottom (left), Cory used in the center and Aci used in the right. Probes are specific for species in the SBBR process

a b c d Fig. Hybridization results of biofilms (8 week) from the SBBR system. (a) Eub 338 (b) Alc ( c ) Cory and (d) Aci probes used in each biofilm slide. Hybridization was carried out at 45 ℃, 3 h.

Table Bacterial levels in SBBR processes with four organic loading by FISH

Table The relationship between community compositions and treatment efficiency* of the SBBR system The COD and TN removal was increased from 79% to 95% and 68% to 92%, respectively The counts for heterotrophic bacteria were also increased during the observation period

Table Effect of shock loading on the bacterial community and treatment efficiency in the SBBR processes Decreasing heterotrophic nitrifying bacteria population by COD variation of influent

Table The bacterial community in the stable SBBR processes operated with four organic loading

l 模擬配水系統 膜片 Robbins devices

生物膜細菌族群分析 採樣 日期* 菌量** (%) Aci Sphin Acido Eub 338 5/16 6. 9± 93% 5. 8± 151% 3. 4± 111% 67. 6± 18% 5/18 12. 4± 131% 6. 5± 74% 5. 2± 107% 68. 9± 17% 5/20 15. 8± 61% 6. 8± 73% 4. 8± 96% 61. 3± 29% 5/22 11. 4± 96% 4. 9± 88% 5. 7± 102% 65. 0± 22% 5/24 8. 2± 53% 4. 0± 68% 6. 2± 63% 71. 1± 16% 6/5 4. 5± 46% 2. 3± 133% 4. 3± 131% 49. 4± 45% 6/6 5. 7± 115% 3. 3± 87% 2. 9± 109% 68. 0± 15% 6/7 4. 8± 63% 5. 8± 72% 1. 2± 160% 66. 2± 25% 6/8 5. 3± 73% 2. 4± 75% 2. 4± 111% 67. 3± 23% 6/9 7. 2± 68% 6. 2± 133% 6. 1± 132% 62. 5± 19% *5/16 – 5/24為水力停留時間 2天； 6/5 – 6/9為水力停留時間 1天 ** FISH菌數/DAPI染色菌數；各數值為觀察視野(n = 49)之平均值，( )內為標準偏差百分比

n 配水系統生物膜樣本測得Legionella sp. ，但濃度不高 n Legionella pneumophila不存在 n 溫度與Legionella sp. 生長之關係

n生物膜樣本中測得Mycobacterium sp. ，屬非致病性及快長菌 n餘氯濃度無法有效抑制Legionella sp. 與Mycobacterium sp. n. DOC含量可提供Legionella sp. 與Mycobacterium sp. 生長

(a) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 (b) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 400 bp 圖 模擬配水系統生物膜中Mycobacterium sp. 與致病性Mycobacterium sp. 檢測 (a)MF/MR引子對 (b)Eub 338/Myc 2引子對