Sequence Comparison Dr Hilal AY Sequence comparison Annotation
- Slides: 21
Sequence Comparison Dr. Hilal AY
Sequence comparison Annotation of new sequences …. Phylogenetic analyses Comparison of nucleotide and protein sequences Building models for proteins Designation of expression profiling experiments
Sequence comparison
Sequence comparison Homologous • Related sequences are designated as being homologous. • Homology is not a measure of similarity, but rather signifies that sequences have a shared evolutionary history and, therefore, possess a common ancestral sequence. Ortholog • Homologous proteins from different species that possess the same function (e. g. , corresponding kinases in a signal transduction pathway in human and mice) are called orthologs. Paralog • Homologous proteins that have different functions in the same species (e. g. , two kinases in different signal transduction pathways of humans) are termed paralogs.
Sequence comparison
Sequence comparison Identity is the ratio of the number of identical amino acids or nucleotides in a sequence to the total number of amino acids or nucleotides. Similarity is not simple to calculate. Before similarity can be determined, the degree of similarity of the building blocks of sequences to each other must first be determined. This is done using similarity matrices that are also known as substitution or scoring matrices. Similarity matrices specify the probability that a sequence will transform into another sequence over time. Of course, this depends on the time elapsed and the mutational rate of nucleotides.
Identical sequences
Identical sequences
Identical sequences
Nucleotide alignment
Nucleotide alignment Identity % ? ? ?
Global alignment – Local alignment
Multiple alignment – nucleotide sequences
Multiple alignment – amino acid sequences
Protein sequences
Protein sequences Codon: GAT / GAC Codon: GAA / GAG Triptofan (Trp) [W] Codon: TGG
Protein sequences Amino acid substitution matrices PAM (position accepted mutation) Block Substitution Matrix
Amino acid alignment
Sequence alignment programs
Sequence alignment programs
Phylogenetic Trees
- Hilal tercan
- Hilal özdağ
- Banou hilal
- Blgen
- Doç dr hilal ay
- Hpv tipleri
- General zaroff first comes to the island after
- What is text annotation
- Scale annotation pipeline
- Ovid metamorphoses icarus
- David functional annotation
- Oh captain my captain imagery
- David go annotation
- Bacteriophage annotation
- Basking shark poem
- Close reading annotation symbols
- Genome assembly and annotation ppt
- Maplex label engine
- Dulce et decorum est annotation
- Morphological annotation
- Shall i compare thee to a summer's day annotation
- O valiant cousin worthy gentleman annotation