Sampling Methods Sampling Purpose Detect and quantify Identify

Sampling Methods

Sampling • Purpose – Detect and quantify – Identify sources/amplification – Assess potential virulence – Assess effectiveness of controls

Cladosporium

Steps in Sampling • • • Selection of a laboratory Selection of analyts Selection of methods Sensitivity of method Field sampling QC Reporting and lab support

Approaches to Sampling • Qualitative - Type • Quantitative - Concentration

Overview of Microbial Sample Collection Methods (Liquid Impinger)

Overview of Microbial Sample Collection Methods (Fungal Spore Sampling & Spore Traps)

Overview of Microbial Sample Collection Methods (Impactor Samplers)

Overview of Microbial Sample Collection Methods (Sedimentation Settling Plates)

Overview of Microbial Sample Collection Methods (Airborne Particulates & Bulk Samples)

Overview of Microbial Sample Collection Methods (Dust & Tape Lifts)

Tape Lift Sampling

Overview of Microbial Sample Collection Methods (Swab/Wipe & Surface Contact Plate)

Overview of Microbial Sample Collection Methods (Water Sample & Endotoxins in air)

Overview of Microbial Sample Collection Methods (Mycotoxins in Air)

Overview of Microbial Sample Collection Methods (MVOC’s in Air & β-1, 3 -D-Glucans in Air)

Overview of Microbial Sample Collection Methods (MVOC’s in Air & β-1, 3 -D-Glucans in Air)

Microbial By Products (Ergosterol in Air) Notes: *Can also analyze for in bulk, surface and water samples. CFU-colony forming units M 3 -cubic metres of air Mg-milligrams Ng-nanograms Ppm-parts per million Ml-milliliter G-gram **References used to general Table 1 above are listed below 1 -8

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Plate Sampling Air Flow Valve Biosis Pump Rotameter

Plate Sampling

Stacked Plate Samplers

Impinger Sampling • Impingement – Liquid media

Impinger Sampling • Types of Impingers – AGI-30 – AGI-4

Impinger Sampling

Air Sampling • Types of Filtration – Membrane filter cassette

Passive Sampling • Deposition – Gravity on agar

Passive Sampling • Types of gravitational – Settling plates

Air Sampling (Spore Trap) • Collects “everything” – Viable, non-viable, pollen, • Unable to differentiate

Carpet Sampling • Collect spores in the carpet pile using air pump • Identifies type of mold • Looking for uncommon types • Provides historical information

Carpet Sampling

Data Interpretation • • • Concentration inside versus outside. Mold types inside versus outside. Presence or absence of a specific mold. Location of unknown source. Change in concentration following remediation. • Is a “snap shot” in time.

Stachybotris

Aspergillus

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References