Ruxolitinib in combination with prednisone and nilotinib exhibit
Ruxolitinib in combination with prednisone and nilotinib exhibit synergistic effects in human cells lines and primary cells from myeloproliferative neoplasms by Alicia Arenas Cortés, Rosa Ayala Diaz, Pilar Hernández-Campo, Julián Gorrochategui, Daniel Primo, Alicia Robles, María Luz Morales, Joan Ballesteros, Inmaculada Rapado, Miguel Gallardo, María Linares, and Joaquín Martínez-López haematol Volume 104(5): 937 -946 April 30, 2019 © 2019 by Ferrata Storti Foundation
Drugs used in the screening to search for the best combination with ruxolitinib. Alicia Arenas Cortés et al. Haematologica 2019; 104: 937 -946 © 2019 by Ferrata Storti Foundation
Effect of each drug in combination with 100 n. M ruxolitinib in BA/F 3 JAK 2 -V 617 F 3 cell line (A) or in patients’ peripheral blood mononuclear cells (B). y-axis: the increment between the EC 50 for each drug in the presence of ruxolitinib minus its EC 50 in monot. . . Alicia Arenas Cortés et al. Haematologica 2019; 104: 937 -946 © 2019 by Ferrata Storti Foundation
Results of the dose-response curves of drugs in monotherapy in patients’ samples after 72 hours of incubation with drugs: median. Alicia Arenas Cortés et al. Haematologica 2019; 104: 937 -946 © 2019 by Ferrata Storti Foundation
Combination Index of ruxolitinib (R), nilotinib (N) and prednisone (P) in samples of cell lines. Alicia Arenas Cortés et al. Haematologica 2019; 104: 937 -946 © 2019 by Ferrata Storti Foundation
Combination Index (CI) of ruxolitinib (R), nilotinib (N) and prednisone (P) in samples of myelofibrosis (MF) patients and healthy donors. Alicia Arenas Cortés et al. Haematologica 2019; 104: 937 -946 © 2019 by Ferrata Storti Foundation
Screening for signaling proteins affected by 32 n. M ruxolitinib (R), 1. 6 μM nilotinib (N), 0. 8 μM prednisone (P), and their combinations, using a phospho-protein array. Alicia Arenas Cortés et al. Haematologica 2019; 104: 937 -946 © 2019 by Ferrata Storti Foundation
Effect of 32 n. M ruxolitinib (R), 1. 6 μM nilotinib (N), 0. 8 μM prednisone (P), and their combinations, on the phosphorylation of ERK 1/2 and STAT 5 in cell lines. Alicia Arenas Cortés et al. Haematologica 2019; 104: 937 -946 © 2019 by Ferrata Storti Foundation
Effect of ruxolitinib (R), nilotinib (N), prednisone (P), and their combination, on collagen I m. RNA expression using quantitative polymerase chain reaction or protein expression by immunocytochemistry. Alicia Arenas Cortés et al. Haematologica 2019; 104: 937 -946 © 2019 by Ferrata Storti Foundation
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