RNA vs DNA 1 RNA Differs from DNA
- Slides: 29
RNA vs DNA 1
RNA Differs from DNA • RNA has a sugar ribose • DNA has a sugar deoxyribose 2
• RNA contains the base uracil (U) • DNA has thymine (T) • RNA molecule is single-stranded • DNA is doublestranded DNA 3
Structure of RNA 4
Three Types of RNA 1. Messenger. RNA (m. RNA) 2. Ribosomal RNA (r. RNA) 3. Transfer. RNA (t. RNA) 5
. • Messenger RNA (m. RNA) copies DNA’s • • code & carries the genetic information to make a protein to the ribosomes Single chain of Nucleotides Made in the Nucleus Leaves through nuclear pores Contains the Nitrogen Bases A, G, C, U ( no T ) 6
• Ribosomal RNA (r. RNA), along with protein, makes up the ribosomes • Made in the nucleus in the nucleolus • Site of Protein Synthesis 7
• Transfer RNA (t. RNA) transfers amino acids to the ribosomes where proteins are synthesized • Single stranded molecule with amino acid at one end • Opposite end has three nucleotide bases called the anticodon 8
PROTEIN SYNTHESIS 9
• DNA contains genes, sequences of nucleotide bases • These genes code for polypeptides (proteins) • Proteins are used to build cells and do much of the work inside cells 10
§ Proteins are made of amino acids linked together by peptide bonds § 20 different amino acids exist 11
Starting with DNA • DNA ‘s code must be copied by RNA and taken to the cytoplasm • In the cytoplasm, this code must be read so amino acids can be assembled to make polypeptides (proteins) • This process is called PROTEIN SYNTHESIS 12
The Genetic Code • A codon is a sequence of 3 bases on the m. RNA that designates an amino acid • AUG – methionine or start codon tells the ribosome to begin making the protein • UAA, UAG, or UGA – stop codons tell it to stop and release the protein 13
The Genetic Code • Use the code by reading from the center to the outside • Example: AUG codes for Methionine 14
Name the Amino Acids • • • GGG? UCA? CAU? GCA? AAA? 15
Transcription and Translation 16
Protein Synthesis § The production or synthesis of polypeptide chains (proteins) § Two phases: Transcription & Translation 17
DNA RNA Protein Nuclear membrane DNA Transcription Eukaryotic Cell m. RNA Ribosome Translation Protein 18
m. RNA Transcription • During transcription, RNA polymerase binds to DNA and separates the DNA strands • RNA Polymerase then uses one strand of DNA as a template to assemble nucleotides into RNA 19
m. RNA Transcription • m. RNA leaves the nucleus through its pores and goes to the ribosomes 20
Question: § What would be the complementary RNA strand for the following DNA sequence? DNA: GCGTATG RNA: CGCAUAC 21
Translation • Translation is the process of decoding the m. RNA into a polypeptide chain • Ribosomes read m. RNA three bases or 1 codon at a time and construct the proteins 22
Translation begins when a m. RNA attaches to a ribosome. As each codon of the m. RNA moves through the ribosome, the proper amino acid is brought to the ribosome by t. RNA. The amino acid is transferred to the growing polypeptide chain. 23
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Each t. RNA carries only one kind of amino acid. Each t. RNA has 3 unpaired bases called an anticodon, that is complementary to one m. RNA codon. 26
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Transcription Translation 29
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