RNA Nucleotide DNA Nucleotide RNA Nucleotide Sugar is
- Slides: 67
RNA Nucleotide DNA Nucleotide
RNA Nucleotide • Sugar is ribose • Bases include: • Adenine • Guanine • Cytosine • Uracil DNA Nucleotide • Sugar is deoxyribose • Bases include: • Adenine • Guanine • Cytosine • Thymine
• Overall Shape: • Double Helix • (looks like a twisted ladder)
• Nitrogen containing bases: • Adenine (A) • Thymine (T) • Cytosine (C) • Guanine (G)
• Base Pairing Rules: • Adenine to Thymine • (A – T) • Cytosine to Guanine • (C to G)
• Backbone: • Phosphate • Sugar (deoxyribose)
• Chargaff’s Rules: • Amount of A always equals amount of T • Amount of C always equals amount of G
§ 2 meters of DNA in each cell of your body §DNA wraps around histone proteins to form chromatin §Before cell division, chromatin winds up (condenses) to form chromosomes
RNA Structure § Single stranded § Sugar = ribose § Bases: § Adenine § Cytosine § Guanine § Uracil DNA Structure § Double stranded § Sugar = deoxyribose § Bases: § Adenine § Cytosine § Guanine § Thymine
§ Messenger RNA (m. RNA) § Carries DNA messages from nucleus to ribosome § Ribosomal RNA (r. RNA) § Makes up part of the ribosome § Transfer RNA (t. RNA) § Reads m. RNA and carries the correct amino acid to the ribosome
§ Instructions for making/controlling all things in the cell
§General Description: § Process of making an exact copy of DNA.
§ Process: 1. Original DNA strands separate 2. Complementary nucleotides attach § Use base pairing rules 3. Backbone formed to create new strand § Sugar and phosphates bond together 4. End Result: Two identical DNA molecules § Each has one original strand one new strand
§Identify the structures 1. 2. 3. 4. Original DNA strands Complementary nucleotides DNA Polymerase (enzyme) Newly constructed DNA Strand
§DNA Replication occurs during the Synthesis (S) phase of the cell cycle
§Terminology: § Transcription: Process of copying a DNA message onto an m. RNA molecule. § Translation: Process of translating the message on an m. RNA into a protein. § m. RNA: Carries the DNA message to the ribosome. § t. RNA: reads the m. RNA message and brings amino acids to the ribosome.
§r. RNA: makes up part of the ribosome. §Codon: 3 letter “word” on m. RNA that codes for an amino acid §Anticodon: 3 letter “word” on t. RNA that matches the codon §Ribosome: structure in the cell where proteins are made
1. 2. 3. 4. 5. DNA Transcription m. RNA Translation Protein
§Example: §DNA: §m. RNA: §Amino Acid: TAC CGG AUG GCC Met Ala TAA CGC AUU GCG Ile Ala
§ DNA determines sequence of m. RNA § m. RNA is grouped into “codons” of 3 letters § Each codon corresponds to a specific amino acid § t. RNA brings the correct amino acid to the ribosome § Amino acids link together to make a protein
Transcription Drawing
Translation Drawing
1. Interphase 2. Gap 1 (G 1) Phase § Does normal cell things, Cell grows, makes more organelles 3. Synthesis (S) phase § DNA Replication occurs 4. Gap 2 (G 2) phase § Preparation for cell division
5. Cell Division (M-phase) 6. Mitosis 7. Cytokinesis 8. Prophase 9. Metaphase 10. Anaphase 11. Telophase 12. Cytokinesis
Step 1: Interphase Purpose: Prepare the cell Gap 1 (G 1): § Normal cell things § Cell grows and copies organelles § Synthesis (S): § DNA Replication occurs § Gap 2 (G 2): § Preparing for cell division § § §
Step 2: Mitosis § Purpose: divide the nucleus
§ Prophase: § Chromosomes condense § Nuclear envelope disappears § Spindle fibers form § Metaphase: § Chromosomes line up along equator (middle)
§ Anaphase: § Chromosomes pulled to opposite ends of cell by spindle fibers § Telophase: § Chromosomes uncoil § Nuclear envelope re-appears § Spindle fibers disappear
Step 3: Cytokinesis § Purpose: divide the cell (cytoplasm) § Occurs after telophase when there are two nuclei in the cell
Step 3: Cytokinesis § In plants: § Cell plate forms down middle of cell § Cells break apart
Step 3: Cytokinesis § In animals: § Cell membrane pinches in § Cells pinch apart
Why we need cell division: § Growth § Growing from a single cell to an adult requires more cells
Why we need cell division: § Replace dead/injured cells § Cells must be replaced as they are lost § Example: skin cells constantly shed and must be replaced
Why we need cell division: § Replace cells after illness § Immune responses destroy healthy and sick cells
PMAT §P=Prophase §M=Metaphase §A=Anaphase §T=Telophase
Parent Cell: Interphase • Diploid • Somatic (body) cell • DNA is copied Prophase: • Chromosomes condense • Nuclear envelope disappears • Spindle fibers form
Metaphase: • Chromosomes line up along equator (middle) • Chromosomes attached to spindle fibers Anaphase: • Chromosomes pulled apart by spindle fibers
Telophase: • Chromosomes uncoil • Nuclear envelopes reappear • Spindle fibers disappear
1 st division of Meiosis: • Almost identical to mitosis • Difference = chromatids on homologous chromosomes can switch • Called crossing over
2 nd division of Meiosis: • This division cuts chromosome # in half • Still similar to mitosis • Difference: end up with 4 unique haploid cells
Purpose of Meiosis: • To create gametes or sex cells that are haploid Importance of creating haploid cells: • A gamete has to fuse with another gamete to make a new individual (fertilization) • If each had full set of chromosomes, the new individual would have twice as many chromosomes
How does each sex cell become unique: • Chromosomes line up randomly • Crossing over occurs
Fertilization: • Fusion of gametes to create a new individual
Egg and Sperm each created through meiosis Egg and Sperm combine during Fertilization creates a new individual Egg = Haploid Sperm = Haploid Egg(haploid) + Sperm(haploid) = New Individual Cell (diploid)
Somatic Cells Gametes: • Body Cells • Diploid • Sex Cells (egg or sperm) • Haploid
1. Autosomes • Regular chromosomes • 22 pairs in humans
2. Homologous Chromosomes • A pair of chromosomes • 1 from mom • 1 from dad • Same genes on each one; just different versions
3. Sex Chromosomes • 23 rd or last pair in humans • Determine gender • Females have XX • Males have XY
Diploid Cell: Haploid Cell: • Has 2 copies of each chromosome 1 from mom’s egg • Has 1 copy of each chromosome 1 from dad’s sperm • These are somatic cells • These are gametes Egg for females Sperm for males
Diploid Haploid
1. 2. 3. 4. 5. Mitosis vs. Meiosis Mitosis Meiosis Makes diploid cells 1. Makes haploid cells Creates identical 2. Creates unique cells Makes somatic cells 3. Makes gametes Used to grow & heal 4. Used to create sex cells for reproduction Cell divides once 5. Cell divides twice
Mitosis: Diploid Parent Cell DNA Replication Nucleus then cell divides Diploid daughter cells
Meiosis Part I: Diploid Parent Cell DNA Replication Nucleus then cell divides Diploid daughter cells
Meiosis Part II: Cell divides again Diploid daughter cells from 1 st division Haploid daughter cells
Haploid Diploid Human 23 46 Earthworm 18 36
Egg/Sperm Zygote Maize (corn) 10 20 Dog 36 78
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