Reversion Mutant type Wild type Revertant Reversion Frequency
- Slides: 50
복귀(Reversion) Mutant type이 Wild type으로 전환 (Revertant). Reversion Frequency (복귀 빈도). 102의 leu- 균 주를 최소배지에 배양 : No Colony. 107의 leu- 균 주를 최소배지에 배양 : 50개 Colony. ∴ 50 / 107 = 50 * 10 -7 = 5 *10 -6. Frequency value(복귀빈도 치) : 5 * 10 -6. 만약 이중돌연변이체의 복귀 시 복귀빈도 치 : 5 * 10 -6)2 = 25 * 10 -12 = 2. 5 * 10 -11.
돌연변이체의 이용 2. 돌연변이체는 생화학적 차단을 통해서 대사경로를 밝힐 수 있다. gal. K gal. E gal. T Gal-1 -p UDP-Gal X(UDP-Glu). 14 C Galactose 첨가 후 20분 마다 반응물 조사. 14 C Galactose-1 -Phosphate, 14 C Uridine diphospho galactose, Uridine diphospho glucose로 전환. If) gal K- : 세포 내 14 C Galactose만 검출. gal T- : 14 C Galactose-1 -phosphate도 검출. gal E- : 14 C Uridine diphospho galactose도 검출. 14 C
삼 인자 교차 법 : Three Factor Cross
상보성 시험 : Complementation test E. coli 감염하는 T 4 phage : coli B 균 주와 K 균 주에서 정상 plaque 형성. E. 돌연변이 T 4 phage : 생활주기 빨라지거나 큰 plaque (r 형) 형성. T 4 phage\대장균 E. coli B E. coli K Wild type 정상 plaque rⅠ mutant r형 plaque rⅡ mutant r형 plaque No Growth(-) rⅢ mutant r형 plaque 정상 plaque
대장균의 유전체계 : F+, FDonor (웅성: Male), Recipient (자성: Female), F plasmid (Sex plasmid : 성 plasmid).
Hfr : High frequency recombinant F+, F-, Hfr, F’ (4 type) 있음.
Plasmid DNA 란? 1. Extrachoromosomal DNA. 2. Doule strand DNA (ds DNA). 3. Covalently Closed Circle form (CCC-form). 4. Replicon. 5. Gene marker. 6. F, R, Col, Ti, 2 ㎛, Tol.
접합 : Conjugation
접합성 Plasmid의 전염성 : Conjugated plasmid
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