Result 5 5 Ether Acetone Result 6 4

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Result (사진) 5: 5 (Ether : Acetone)

Result (사진) 5: 5 (Ether : Acetone)

Result (사진) 6: 4 (Ether : Acetone)

Result (사진) 6: 4 (Ether : Acetone)

Result (사진) 7: 3 (Ether : Acetone)

Result (사진) 7: 3 (Ether : Acetone)

Result (사진) 8: 2 (Ether : Acetone)

Result (사진) 8: 2 (Ether : Acetone)

Result (사진) 9: 1 (Ether : Acetone)

Result (사진) 9: 1 (Ether : Acetone)

Result (Rf 값) 5: 5 빨간 장미 노랑 장미 흰색 장미 잎 0. 97

Result (Rf 값) 5: 5 빨간 장미 노랑 장미 흰색 장미 잎 0. 97 0. 96 0. 65 0. 20 0. 94 6: 4 0. 97 0. 44 0. 98 7: 3 0. 97 0. 15 0. 56 0. 97 0. 14 0. 47 0. 97 0. 06 0. 14 0. 57 0. 99 0. 80 0. 69 0. 80 0. 99 8: 2 0. 72 0. 12 0. 49 0. 08 0. 05 0. 23 0. 64 0. 41 0. 61 0. 97 0. 98 9: 1 0. 32 0. 05 0. 16 0. 11 0. 74 0. 95 0. 94

Reference http: //user. chol. com/~hl 5 nol/thin. htm http: //blog. naver. com/lyceum 1000? Redirect=Log&log.

Reference http: //user. chol. com/~hl 5 nol/thin. htm http: //blog. naver. com/lyceum 1000? Redirect=Log&log. No=4002 9054560 http: //kin. naver. com/detail. php? d 1 id=11&dir_id=110204 &eid=Xssg. GYb 6 YAZnl. Hp. Fgj. F 1 y. Jy. G 6 t. Yh 6 E+f&qb=7 Je. Q 7 Yu 4 7 Je. Q 7 YWM 66 W 0&enc=utf 8&pid=fm 61 hwoi 5 UNssvi. L 0 Lhsss --271246&sid=Sh. KJy. Gt 3 Eko. AAHqc. EEI http: //100. naver. com/100. nhn? docid=111187 http: //kin. naver. com/detail. php? d 1 id=11&dir_id=110204 &eid=0 Jvmvj. GLg 5 ha. NHp. W 6 aza+h 8 z. RMrik. Th 1&qb=7 YGs 66 Gc 66 e. I 7 Yag 6 re 4656 Y 7 ZS 867 a. E 66 as 7 LCo 7 J 20&enc=utf 8

Bio Reagents and Chemicals • • Reagents needed CTAB buffer 2% CTAB 20 gm

Bio Reagents and Chemicals • • Reagents needed CTAB buffer 2% CTAB 20 gm CTAB 20 m. M EDTA 40 ml EDTA stock (0. 5 M) 100 m. M Tris-Cl p. H 8. 0 100 ml Tris-Cl stock (1 M) 1. 4 M Na. Cl 280 ml Na. Cl stock (5 M) make up to 1 Litre with water, p. H 7. 5 - 8. 0, and autoclave + 0. 2% Mercaptoethanol • • • Wash Buffer 76% Ethanol 10 m. M NH 4 A

Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction 1. Preheat 5 ml CTAB (add 10µl mercaptoethanol

Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction 1. Preheat 5 ml CTAB (add 10µl mercaptoethanol to each 5 ml CTAB) in a blue-topped 50 ml centrifuge tube at 6065℃. Remove and discard midribs, and wrap laminae in aluminium foil and freeze in liquid nitrogen. 0. 5 – 1. 0 gm tissue/5 ml CTAB (Can store leaf material after liquid Nitrogen – 1 -2 days at – 20 or – 80 for longer periods) 2. Gently crumble leaf tissue over cold pestle of liquid nitrogen. Grind frozen leaf with one spatula of fine sand add 0. 5 spatula of PVPP powder after grinding. 3. Scrape powder into dry tube and add pre-heated buffer and mix gently. Avoid leaving dry material around rim of tube. Adjust CTAB volume to give a slurry-like consistency, mix occasionally. 4. Incubate for 60 min at 60℃ 5. Add equal volume of chloroform/iso-amyl alcohol (24: 1), Mix for about 3 min, then transfer contents to narrow bore centrifuge tubes. Balance by adding extra chlor/iso. Spin 5, 000 rpm for 10 min (ensure correct tubes used), brake off. (For extra pure DNA isolation - spin and retain supernatent before chloroform extraction).

Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction 6. Remove supernatant with wide-bore pastette (cut off

Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction 6. Remove supernatant with wide-bore pastette (cut off blue tip) to clean tube, repeat chloroform extraction once. Supernatent should be clear, though may be coloured. 7. Precipitate DNA with 0. 66 vol. of cold isopropanol can leave overnight. Spool out or spin down DNA, 2 min at 2, 000 rpm. 8. Transfer to 5 ml wash buffer for 20 min. 9. Dry briefly and resuspend in 1 ml T. E. (can be left overnight) 10. Add 1µl 10 mg/ml RNAse to each 1 ml T. E. /DNA mixture and incubate for 60 min at 37℃. (If RNASe in the sample doesn’t matter – stages 11 and 12 may be omitted) 11. Dilute with 2 volumes T. E. and add 0. 3 vol 3 M Sodium acetate (p. H 8) + 2. 5 vol cold 100% ethanol, 12. Spool DNA out. Air dry and resuspend in in 0. 5 to 1 ml T. E. (takes time) and freeze until required

Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction CTAB method 순서의 요약. CTAB 의 구조식

Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction CTAB method 순서의 요약. CTAB 의 구조식

Reference http: //kin. naver. com/detail. php? d 1 id=11&dir_id=110205& eid=G 0 i. Za. XWa.

Reference http: //kin. naver. com/detail. php? d 1 id=11&dir_id=110205& eid=G 0 i. Za. XWa. E 6 C 8 ONR 04 Sk 3 p 3 lm 9 MN 0 PObo&qb=Y 3 Rh. Y g==&enc=euc-kr&order=3 http: //en. wikipedia. org/wiki/Hexadecyltrimethylammonium_bro mide http: //blog. naver. com/hongyifan 78? Redirect=Log&log. No=6005 0111735 03학번 이성남 선배(서울여대 박사과정)에게 질문을 통해 받은 답. http: //en. wikipedia. org/wiki/File: Cetrimonium_bromide. png 유전학실험서 (라이프 사이언스 출판 한국유전학회 지음 P. 129)