RESPIRATORY ENZYMES ATP Catalaze Superoxide dismutase O 2

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RESPIRATORY ENZYMES ATP Catalaze Superoxide dismutase O 2 1 H 20 2 Amal Ali

RESPIRATORY ENZYMES ATP Catalaze Superoxide dismutase O 2 1 H 20 2 Amal Ali ALmousa 140 mic 02/02/43

Anaerobies Aerobes Microaerophiles Obligate aerobes Facultative anaerobes Obligate anaerobes Aerotolrant anaerobes 2 Amal Ali

Anaerobies Aerobes Microaerophiles Obligate aerobes Facultative anaerobes Obligate anaerobes Aerotolrant anaerobes 2 Amal Ali ALmousa 140 mic 02/02/43

Obligate aerobies 3 Obligate anaerobes Facultative anaerobes Amal Ali ALmousa 140 mic Microaerophiles Aerotolrant

Obligate aerobies 3 Obligate anaerobes Facultative anaerobes Amal Ali ALmousa 140 mic Microaerophiles Aerotolrant anaerobes 02/02/43

Obligate anaerobes in lab 4 Amal Ali ALmousa 140 mic 02/02/43

Obligate anaerobes in lab 4 Amal Ali ALmousa 140 mic 02/02/43

Balanced growth Growth cycle: Lag phase Log phase Stationary phase Death phase The Growth

Balanced growth Growth cycle: Lag phase Log phase Stationary phase Death phase The Growth Curve 5 Amal Ali ALmousa 140 mic 02/02/43

Measuring Growth 1 -Cell Count • Direct Measurement Direct Microscopic Count hemocytometer In direct

Measuring Growth 1 -Cell Count • Direct Measurement Direct Microscopic Count hemocytometer In direct Measurement colony counting 6 Amal Ali ALmousa 140 mic 02/02/43

2 -Mass determination Direct Measurement weighed (wet mas). dried weighed( dry mass In direct

2 -Mass determination Direct Measurement weighed (wet mas). dried weighed( dry mass In direct Measurement )Turbidity 3 -Measurement of enzymatic activity In direct Measurement 7 Amal Ali ALmousa 140 mic 02/02/43

Factors affecting microbial growth 1 -Physical factors 2 -Chemical factors 3 -Biological factors 8

Factors affecting microbial growth 1 -Physical factors 2 -Chemical factors 3 -Biological factors 8 Amal Ali ALmousa 140 mic 02/02/43

1 -Physical factors Temperature O 2 p. H Osmotic Pressure radiation Moisture Desication Surface

1 -Physical factors Temperature O 2 p. H Osmotic Pressure radiation Moisture Desication Surface tension atmospheric pressure 9 Amal Ali ALmousa 140 mic 02/02/43

1 -Temperature • Minimum growth temperature – Optimum growth temperature – Maximum growth temperature

1 -Temperature • Minimum growth temperature – Optimum growth temperature – Maximum growth temperature – 10 Amal Ali ALmousa 140 mic 02/02/43

2 -Oxygen concentration slid 2 3 - p. H value Acidophilus : p. H

2 -Oxygen concentration slid 2 3 - p. H value Acidophilus : p. H 0 and 5. 5 Neutrophilus : p. H 5. 5 and 8 Alkalophileus : p. H 8 – 11. 5 11 Amal Ali ALmousa 140 mic 02/02/43

4 -Osmotic Pressure: A-Hypotonic B-Hypertonic Plasmolysis C-Isotonic 12 Amal Ali ALmousa 140 mic 02/02/43

4 -Osmotic Pressure: A-Hypotonic B-Hypertonic Plasmolysis C-Isotonic 12 Amal Ali ALmousa 140 mic 02/02/43

5 - radiation(UV rays, Ionizing radiation ) 6 -Moisture (Water activity) 7 -Desiccation 13

5 - radiation(UV rays, Ionizing radiation ) 6 -Moisture (Water activity) 7 -Desiccation 13 Amal Ali ALmousa 140 mic 02/02/43

8 -Surface tension 9 - atmospheric pressure 14 Amal Ali ALmousa 140 mic 02/02/43

8 -Surface tension 9 - atmospheric pressure 14 Amal Ali ALmousa 140 mic 02/02/43

2 -Chemical factors 1 -Germistatic agents 2 -Germicidal agents 15 Amal Ali ALmousa 140

2 -Chemical factors 1 -Germistatic agents 2 -Germicidal agents 15 Amal Ali ALmousa 140 mic 02/02/43

3 -Biological factors Microbes group 16 Amal Ali ALmousa 140 mic S N A

3 -Biological factors Microbes group 16 Amal Ali ALmousa 140 mic S N A L E R O I T 02/02/43

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