Requirement of animal cell culture B Sc III

Requirement of animal cell culture B. Sc. III Dr. Narayan Mane Department of Zoology GKG College Kolhapur

• The need of animal cell culture is a quite different from biology, molecular biology and biochemistry laboratory but similar to the microbiology, virology laboratory. • For animal cell culture laboratory require following three factors- 1. Equipments and materials. 2. Culture medium 3. Animal cell culture laboratory.

Equipments and materials

Laminar air flow Horizontal laminar air flow Vertical Laminar air flow

Autoclave

Inverted phase contrast microscope

Water purifier

centrifuge

Incubator

Hot air oven

Deep freezer

Water bath

Balance

p. H meter

Magnetic stirrer

Vortex mixture

Osmometer

Aspirator jar

Haemocytometer

CO 2 incubator

Liquid nitrogen storage

Plastic and glass wears

T-Flask Multiwell plate Round bottom culture flask IVF plate

Slide chamber Cell scraper Cell strainer Falcon tubes

Ependoff tubes Centrifuge tubes Tube rack Transparent pipette

Disposable pipette Pipette controller Micro pipette tips

Cryo tubes Surgical instrument Syringe filter Aluminum foil

Surgical mask Surgical cap Surgical gloves Surgical gown

p. H of medium Inorganic salts Antibiotics Oxygen Serum Tissue culture medium Components and properties Trace element Amino acids Carbohydrates Fatty acids and lipids Proteins and peptides

Type of tissue culture medium Natural medium Artificial medium Commonly used medium 1. Serum containing medium 1. EMEM 2. Serum free medium 2. DMEM 3. RPMI 4. Hams nutrient medium 3. Chemically defined medium 4. Protein free medium

Tissue culture laboratory

Culture room Storage area Washing area