Reprogramming of somatic cells via TAT mediated protein

Reprogramming of somatic cells via TAT- mediated protein transduction of recombinant factors Presented by Wu Meng

Induced Pluripotent Stem Cells

Safe Virus 2006 Plasmids 2007 Proteins 2009 m. RNA 2010

Protein Transduction Domain PTD-PTD Construct Expression Transduction Target gene PTD-target gene fusion protein Add to cell culture medium Injection

PTD Mediated Protein Delivery PTD

TAT: Powerful in vivo protein transduction system

TAT: Powerful in vivo protein transduction system HIV-1 TAT PTD Tyr-Gly-Arg-Lys-Arg-Gln-Arg-Arg Fluorescence confocal microscopy of hemispheric sagittal brain sections (top) and skeletal muscle tissue sections (bottom) isolated from mice 20 min after ip injection. Steven F. Dowdy Science , 1999

Characterization of TAT-mediated transduction of RFs

TAT PTD and 11 R mediated RF transduction

Transduced TAT-RFs and 11 R-RFs are transcriptionally active

TAT-RFs are functionally active in reprogramming (Mouse NPC) TAT-Sox 2 (n. M) 25 50 Virus O/K/M 75

TAT-RFs are functionally active in reprogramming (Human HFFs)

Reprogramming of human foreskin fibroblast cells using recombinant TAT-RFs.

Significiency Detailed characterization of recombinant RFs in reprogramming Quicker process and higher efficiency than current protocl To facilitate the generation of clinically useful and genetic material-free human i. PSCs.