Replicative senescenceassociated gene expression changes in mesenchymal stromal Slides: 7 Download presentation Replicative senescence-associated gene expression changes in mesenchymal stromal cells are similar under different culture conditions by Katharina Schallmoser, Christina Bartmann, Eva Rohde, Simone Bork, Christian Guelly, Anna C. Obenauf, Andreas Reinisch, Patrick Horn, Anthony D. Ho, Dirk Strunk, and Wolfgang Wagner haematol Volume 95(6): 867 -874 May 31, 2010 © 2010 by Ferrata Storti Foundation Growth curves of MSC were cultured in α-MEM supplemented with either 10% FBS or 10% p. HPL. Katharina Schallmoser et al. Haematologica 2010; 95: 867874 © 2010 by Ferrata Storti Foundation Morphological analysis of MSC were cultured in medium with either 10% p. HPL or 10% FBS as indicated. Katharina Schallmoser et al. Haematologica 2010; 95: 867874 © 2010 by Ferrata Storti Foundation Gene expression changes in MSC upon large scale expansion. Katharina Schallmoser et al. Haematologica 2010; 95: 867874 © 2010 by Ferrata Storti Foundation Gene. Onthology analysis of senescence-associated genes in FBS or p. HPL medium. Katharina Schallmoser et al. Haematologica 2010; 95: 867874 © 2010 by Ferrata Storti Foundation Gene expression changes in long-term culture of different MSC preparations. Katharina Schallmoser et al. Haematologica 2010; 95: 867874 © 2010 by Ferrata Storti Foundation Gene expression changes are continuously acquired during long-term culture. Katharina Schallmoser et al. Haematologica 2010; 95: 867874 © 2010 by Ferrata Storti Foundation Chapter 17: from gene to proteinHikmet hassaSeks kordUndifferentiated mesenchymal cellsUndifferentiated mesenchymal cellsRiñonAlectinibConnective tissue fiber