Renewal Animal Training UConn Health 2020 Renewal Animal

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Renewal Animal Training UConn Health 2020

Renewal Animal Training UConn Health 2020

Renewal Animal Training �This annual renewal training is being implemented �To remind you of

Renewal Animal Training �This annual renewal training is being implemented �To remind you of important considerations when working with animals and in any UConn Health (UCH) animal facility or laboratory �To keep you up-to-date with various federal and local laws, regulations, and policies �To document your adherence to the IACUC protocol and federal and local laws, regulations, and policies

What’s new? �Implementation of Info. Ed for protocol development and review as well as

What’s new? �Implementation of Info. Ed for protocol development and review as well as facility management. �If you have any issues, please contact the OVPR IT helpdesk at 860 -486 -7944. Please DO NOT contact the IACUC office or CCM office with technical problems with Info. Ed.

Protocol Compliance �The following slides are going to explain the most common reasons that

Protocol Compliance �The following slides are going to explain the most common reasons that UCH had to report violations of Public Health Service (PHS) Policy to the Office of Laboratory Animal Welfare (OLAW), NIH for the past year or to the USDA/APHIS �Please think of these slides as “What Not To Do” �If you have any questions, please contact the IACUC office at ooacc@uchc. edu

Reports to OLAW � 2017, we had 15 �In 2018, we had 7 �In

Reports to OLAW � 2017, we had 15 �In 2018, we had 7 �In 2019, we had 2 �Biggest non-compliant items: �Analgesics not given as described in the approved protocol �Procedures being performed on animals that are not described in the protocol �Use of expired drugs/materials in animals

Reports to OLAW �Protocol suspensions. Any protocol or animal activity suspension is an automatic

Reports to OLAW �Protocol suspensions. Any protocol or animal activity suspension is an automatic report to OLAW and USDA (if the species used is a USDA regulated species). We had no suspensions of protocols for the 2019 year. �Most times, the non-compliance which resulted in a report to OLAW can be traced back to an individual not reading the protocol. �If, after reading the protocol, you are ever in doubt as to whether or not you can (or cannot) do an activity in a laboratory animal, contact the IACUC office.

Reports to USDA/APHIS �In 2019, we had no reports to USDA/APHIS �In 2019, we

Reports to USDA/APHIS �In 2019, we had no reports to USDA/APHIS �In 2019, we had no citations from USDA/APHIS on any inspection site visit

Protocol Withdrawals �The IACUC will withdraw protocols that do not have an annual review

Protocol Withdrawals �The IACUC will withdraw protocols that do not have an annual review submitted by the deadline �This means that all work on the protocol must cease �This means that no breeding can be done on an withdrawn protocol – breeders must be separated �Protocols will not be re-instated until an annual review has been submitted and approved by the IACUC

Personnel Withdrawals �Individuals will be removed from protocols: �If they fail to complete renewal

Personnel Withdrawals �Individuals will be removed from protocols: �If they fail to complete renewal training by the due date given to them by the IACUC office; �If they fail to re-enroll in the OHS program by the due date given to them by the IACUC office �Individuals removed from protocols will also have their access to the vivarium removed �PIs will need to modify all protocols to re-add individuals if they have been removed �Individuals will need to re-request facility access from CCM if their facility access has been removed

Protocol Approvals �The IACUC is requiring that all protocols – both animal care and

Protocol Approvals �The IACUC is requiring that all protocols – both animal care and use protocols and animal safety protocols – be signed by all individuals listed on the protocols. This signature is to assure that each individual: �Has read the protocol; �Understands the protocol; and �Will follow the protocol

Important Reminders �The next few slides are going to inform you of important items

Important Reminders �The next few slides are going to inform you of important items of which you need to be aware when you are using laboratory animals at UCH.

Humane Endpoints �All animal care and use protocols require the listing of humane endpoints.

Humane Endpoints �All animal care and use protocols require the listing of humane endpoints. �CCM SOPs that cover humane endpoints can be found at https: //ovpr. uchc. edu/services/rics/animal/ccm/. �IACUC policies that discuss humane endpoints and other appropriate guidelines can be found at https: //ovpr. uchc. edu/services/rics/animal/iacuc/policies/. �Not knowing about any policy or guideline is not an excuse for not following them. It is YOUR responsibility to be familiar with all policies and guidelines at UConn Health.

Body Condition Scoring �The IACUC is encouraging the use of Body Condition Scoring as

Body Condition Scoring �The IACUC is encouraging the use of Body Condition Scoring as a way to evaluate rodents to ensure humane endpoints. �Please arrange training from Veterinary Services if this is new for you. We will now include this in animal care and use protocols in the near future.

Transgenic/Gene Targeted Rodents �It is a requirement at UCH to have Genetically Modified Organism

Transgenic/Gene Targeted Rodents �It is a requirement at UCH to have Genetically Modified Organism (GMO) numbers for all transgenic / gene targeted rodents regardless of their source of origin. This includes rodents made via CRISPR/Cas 9. �These numbers are generated to ensure compliance with both animal welfare regulations and recombinant DNA regulations. �These numbers can only be generated by the IBC coordinator and the IACUC coordinator. Do not make up your own GMO numbers. �The full name of the transgenic animal, a nickname identified in the protocol, or the GMO number must be on all cages that contain genetically modified rodents.

Protocol Research Staff �Everyone who uses animals must be listed on the approved protocol

Protocol Research Staff �Everyone who uses animals must be listed on the approved protocol �When people leave your laboratory and/or are no longer working on your protocol, you need to modify your protocol to delete personnel �In order to use animals, everyone must: �Have completed initial or renewal training �Be current in OHS enrollment �Be listed on an approved protocol �Have clearance from Employee Health Service (x 2893)

Procedure Training �Everyone that works on an animal protocol must document all animal procedural

Procedure Training �Everyone that works on an animal protocol must document all animal procedural training received �It is the PI’s responsibility to ensure procedure training; it is the staff member’s responsibility to document this training �The training document is on the IACUC website at https: //ovpr. uchc. edu/wpcontent/uploads/sites/2568/2015/08/IACUCtrainingrecordsf orm 1. docx �These documents may be obtained during semi-annual lab inspections, asked for during an annual review, or whenever the IACUC requests such documentation

Procedure Training �Consider training from Veterinary Services �They can train you on most procedures

Procedure Training �Consider training from Veterinary Services �They can train you on most procedures performed at UConn Health �You may be required by the IACUC to have training from Veterinary Services �You still need to document your training received from Veterinary Services (see previous slide) �If you need refresher training on how to do something, please call them. They will help you.

Procedure Training �How do I know if I have a safety protocol associated with

Procedure Training �How do I know if I have a safety protocol associated with my animal protocol? � The animal protocol will state it in the hazards section of the protocol. As everyone is required to read the animal protocol, there should be no questions as to whether or not you are working with a chemical or biological hazard that requires appropriate containment. �Animals will be housed in the appropriate hazard facility as specified in your safety protocol(s) You must give CCM 3 days notice before starting any work with hazards that require containment in the chemical isolation room, the chemical isolation/ABSL-2 room, or the ABSL-2 suite. � Training for the use of hazard rooms requires documentation. This documentation of training MUST be forwarded to the IACUC office. �

Name of Core facility (Room #) Chem. Iso (B 5006) Chem. Iso/ABSL-2 (B 5008)

Name of Core facility (Room #) Chem. Iso (B 5006) Chem. Iso/ABSL-2 (B 5008) ABSL-2 (B 6008 A, B 6006, B 6010, B 6008) Traffic Pattern 1. 2 4 4 Air Flow Both room and cage racks are negative Hazard(s) Hazardous Chemicals ONLY; no Biohazards Hazardous Chemicals and Biohazards ONLY; no Hazardous Chemicals Purpose/Function a) a) a) b) Housing for animals being administered hazarous chemicals. Capture of chemical vapors and contaminated particles from bedding and during procedures. Procedures involving animals and hazardous chemicals and cage dumping performed inside the BSC. b) c) Biohazard (ABSL-2) housing/containment for animals being administered hazardous chemicals while in containment. Capture of chemical vapors, contaminated/infectious particles during manipulations. Procedures involving animals and hazardous chemicals and infectious agents in BSC Euthanasia behind empty cage rack b) c) Biohazard (ABSL-2) housing/containment for animals being administered infectious agents or human materials. Capture of infectious liquid and dry particles from bedding and during procedures. Procedures involving infected animals and infectious agents in BSC Euthanasia in ABSL-2 procedure room Engineering Controls: BSCs/cage blowers- recertified annually - learn correct usage (sash at mark) Class II type B 1 Biological Safety Cabinet (BSC). HEPA filtered air exhausted into dedicated hazard- rated duct to the roof. Cage dumping station in the work surface of the BSC. Class II type A 2 Biological Safety Cabinet (BSC). HEPA filtered air exhausted into the room. Rule for housing in each core facility: Mice must be housed in Chem. Iso until chemical has cleared. May move to regular 1. 2 housing in clean cage after chemicals clear, as determined by risk assessment – X many days (below) after last administration of chemical hazard. No other housing; once entered, mice must be housed in Chem. Iso/ABSL-2 for the duration of the experiment. No other housing; once entered, mice must be housed in ABSL-2 for the duration of the experiment. Temporarily leaving the housing core: Mice may be taken to the lab or other core facilities. Clean up any animal excreta as a chemical spill until after chemicals clear the animals. See your safety protocol. Mice return to Chem. Iso for housing until after chemicals have cleared and they are moved to regular housing. Infected mice may be taken to other ABSL-2 (CT DPH registered) rooms only, where containment must be maintained throughout transport and during use outside the facility. See your safety protocol. Pre-transition chem-only mice in cages may go to non-DPH rooms after cage disinfection but must return to Chem. Iso/ABSL-2 for housing. Infected mice may be taken to other ABSL-2 (CT DPH registered) rooms only, where containment must be maintained throughout transport and during use outside the facility. See your safety protocol. Mice must return to ABSL-2 for housing. The door between B 5006 and B 5008 Do not use. Not applicable. Labeling active cages (containing mice) Clear cage card with hazardous chemical label; label says names of chemicals and Haz#s. Clear cage card with bio and chem hazard labels; labels say names of bio/chem hazards and Haz#s. Clear cage card with biohazard labels; labels say names of infectious agents (biohazards) and Haz#s. Dirty cages without mice (storage until dumping) Put on ventilated empty cage rack; leave clear cage card with hazard labels on the cage. Put on empty cage rack in the ante room (B 6008 A); clear cage card with hazard labels left on the cage. Cage dumping Dump station in ducted BSC in B 5008. Dump station in ducted BSC in B 5006. Enclosed empty cages transported to CCM auto-clave. Post sterilization, to regular cage dumping. CO 2 Euthanasia (Euthanex) In B 5005 A; move to euthanize and return cage to B 5006 for storage/dumping. In Chem. Iso/ABSL-2 (B 5008) on far side of dirty cage storage ventilated cage racks. In ABSL-2 Procedure Room (B 6008). Carcasses Double bagged and labeled, outside bag clean of chemicals. Label: Haz# and name of chemical on a chem hazard label. Carcasses from Chem. Iso go for hazardous incineration via RMW burnbox marked for incineration. Interim storage in LB 003. Double bagged and labeled, outside bag disinfected before removal from BSC. Labels: Haz#s and name of chemical on a chem hazard label and name of biohazard on a biohazard label. Carcasses go for hazardous incineration via marked RMW burnbox. Double bagged and labeled, outside bag disinfected before removal from BSC. Label: Haz# and name of biohazard on a biohazard label. Carcasses go for hazardous incineration via RMW burnbox marked for incineration. Interim storage in B 6008 or LB 003. PPE; Full PPE = 1 x gloves; 1 x booties; gown; head cover; mask or respirator; eye/face protection outside the BSC. Full PPE with double gloves/booties; to exit, remove outer booties as cross the threshold out of B 5008 into the hall. Remove all but inner gloves and inner booties outside door in hall; removing outer gloves last. Remove inner booties and inner gloves (last) at outer gowning station. Full PPE with double gloves/booties; to exit, remove all but inner gloves and inner booties in the ante room, removing outer gloves last. Remove inner booties and inner gloves (last) at outer gowning station. Transition from both rooms Chem. Iso/ABSL -2 to divided Chem. Iso and Chem. Iso/ABSL-2 (5 th Floor Only) Room will be emptied, disinfected and then only new Chem-only experiments may be housed in B 5006. All existing experiments from both rooms remain in Chem. Iso/ABSL 2 (B 5008) until existing mice in the experiment are euthanized. No transition; this suite is not involved in the changes on the fifth floor. Chemical Clearance Times: Tamoxifen: 10 days Streptozotocin: 2 days Others: TBD Tamoxifen: N/A Streptozotocin: N/A Others: N/A No chemicals allowed

In the Animal Facility � Traffic flow patterns must be followed � Traffic patterns

In the Animal Facility � Traffic flow patterns must be followed � Traffic patterns can start at 1. 0 and go through 8. 0 � Start out at your lowest traffic pattern. You can go higher in the same day, but not lower so be sure to plan your work accordingly � PPE � You MUST wear appropriate PPE in the facility. Signs on doors state what PPE is required. You must follow it. � Remember, long pants and closed-toe shoes are part of your PPE. � Doors into animal facility hallways � Signs state doors must be kept closed. Please adhere to this requirement. �Music � You are not allowed to play music in the animal facility through speakers. Music with headphone is allowed as long as the volume is low and the music cannot be heard in the room.

USDA Regulated Species �Special attention is required when using USDAregulated species. �Individuals using USDA-regulated

USDA Regulated Species �Special attention is required when using USDAregulated species. �Individuals using USDA-regulated species must contact Veterinary Services (veterinaryservices@uchc. edu) for regulated species training. �Remember- USDA-regulated species are warm blooded vertebrate animals with the exception of mice (genus Mus) and rats (genus Rattus) that have been bred/used for research purposes

Important Things to Remember �Survival surgery requires the use of surgical gloves – major

Important Things to Remember �Survival surgery requires the use of surgical gloves – major or minor, it does not matter. If animals will survive, sterile surgical gloves must be used. �No loud music is allowed in the vivarium. You can use earbuds but if anyone else can hear it, it is too loud. �Drug mixtures must have the appropriate expiration date. They cannot exceed 3 months from the date the mixture is made. If any drug used in the mixture expires sooner than 3 months, the soonest expiration date governs.

IACUC Policies �Please review the IACUC policies. They can be found on the IACUC

IACUC Policies �Please review the IACUC policies. They can be found on the IACUC website at https: //ovpr. uchc. edu/services/rics/animal/iacuc/poli cies/ �It is expected that ALL animal users will be compliant with all IACUC policies �Failure to comply with IACUC policies can result in sanctions from the IACUC up to, and including, protocol suspension or suspension to use animals.

Compassion Fatigue �Compassion Fatigue symptoms are normal displays of stress resulting from the care

Compassion Fatigue �Compassion Fatigue symptoms are normal displays of stress resulting from the care giving work you perform on a regular basis. �Symptoms might include �Excessive blaming �Bottled up emotions �Isolation from others �Remember �Compassion fatigue is synonymous with secondary traumatic stress �Compassion fatigue is not burnout �Some symptoms of compassion fatigue mimic PTSD

Compassion Fatigue �If you feel like you may be having symptoms of Compassion Fatigue,

Compassion Fatigue �If you feel like you may be having symptoms of Compassion Fatigue, there is help available. Talk with �Your supervisor �Occupational Medicine (x 2893) �Employee Assistance Program (x 2877)

Training Documentation � Completion of your annual renewal training signifies your attestation that: �

Training Documentation � Completion of your annual renewal training signifies your attestation that: � You will perform my duties in accordance with the Animal Welfare Act, USDA Regulations (9 CFR), PHS Policy on Humane Care and Use of Laboratory Animals, current AVMA Guidelines for the Euthanasia Animals, and current Guide for the Care and Use of Laboratory Animals; � You will review the protocol(s) under which you will be performing work and will be responsible for conducting this work as it is stated in the approved IACUC protocol(s) [INCLUDING safety protocols]; � You will ensure that IACUC approval has been received before conducting any procedures not listed on the original protocol(s); � You will immediately notify the attending veterinarian, the PI, and the IACUC office when any unanticipated pain/distress or unexpected morbidity/mortality occurs within any of my studies. � Submission of this will complete your annual renewal training requirements for the IACUC at UConn Health. Go to the next page for submission of training completion

Training Documentation �Click HERE to send an email that completes the documentation of this

Training Documentation �Click HERE to send an email that completes the documentation of this training. �If the link above does not work, please send a NEW email (not a reply from an email sent to you) to ooacc@uchc. edu with the following subject line: 2020 Animal User Renewal Training Completed