REMOVAL OF CADMIUM WITH CADMIUM BINDING SURFACE PROTEINS
REMOVAL OF CADMIUM WITH CADMIUM BINDING SURFACE PROTEINS FROM LACTOBACILLUS PLANTARUM Sophie Ivarsson
AIM OF THE STUDY 48 Cd Aim To extract and purify the cadmium binding proteins from the surface of L. plantarum and to evaluate the potential of these proteins as an intravenous therapy to treat cadmium poisoning. Cadmium 112. 411 Cd Cd Cd el Cd Cd B d loo v s es
CADMIUM POISONING AND TOXICITY 48 Cadmium is a heavy metal causing poisoning in humans Half-life: In blood = 128 days In the body = 10 -30 years Current treatments are chelating agents Cd Cadmium 112. 411
LACTOBACILLUS PLANTARUM Gram positive, probiotic bacteria Cadmium reducing properties Cadmium binding surface proteins
HYPOTHESIS Cadmium binding proteins can be purified from the surface of L. plantarum and that these proteins can bind intravenous cadmium. Furthermore, that the proteins will be excreted via the urine or feces.
OVERVIEW Culture bacteria Extraction of proteins Purification of cadmium binding proteins Analysis of structure and characteristics Administratio n intravenous
EXTRACTION AND PURIFICATION OF CADMIUM BINDING SURFACE PROTEINS Culturing bacteria CDM Alternative outcomes… No extraction Extract with buffer containing Li. Cl Purify with IMAC – cadmium column Low protein concentration
STRUCTURE AND CHARACTERISTICS OF PROTEINS No in vitro model Analysis of structure - Mass Spectrometry Analysis of hydrophobicity - Hydrophobic Interaction Chromatography
IN VIVO EXPERIMENTS Induced cadmium poisoning: orally and pulmonary Intravenous administration of cadmium binding proteins Measure cadmium concentrations Cd Cd Alternative outcomes… No excretion
SIGNIFICANCE No good cure today Many affected Why intravenous? v Probiotics only work in intestine v Fast absorption v Pulmonary exposure Utilize the positive properties of the probiotics without being restricted by the limiting properties
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