Relationship between radiation dose and chromosome aberration frequency

Relationship between radiation dose and chromosome aberration frequency – Calibration curves Piotr Knapp Piotr Wróblewski Laboratory of Radiation Biology Supervisors: Ekaterina Zaytseva, Polina Kutsalo, Marta Deperas-Kamińska

The fate of irradiated cell Gy After high dose Correct repair Necrotic death Healthy cell Incorrect repair No effect Somatic effect Cell with damaged DNA Unstable chromosomal aberrations Micronuclei Mitotic death Somatic effect Stable chromosomal Aberrations - Mutations Mutation path Cancer Carcinogenic effect Apoptotic death No effect In the germ cells Genetic effect Somatic effect

Aim of the project �Creation of dose dependency calibration curve of chromosomal aberrations in human peripherial blood lymphocytes exposed to protons beam (LET≤ 100 ke. V/µm) performed at Phasotrone (LNP, JINR) �Biological dosimetry - Allow to assess an accidental radiation dose on base of the level of changes in chromosome structure.

Scheme of the experiment Culture of irradiated blood Brd. U Colcemid Fixation 0 -24 h 48 h e h it gw in n i ta o Flu nc e c s re S Watching s Plu 48 -50 h G s iem a

Karyogram

Chromosomal aberrations

An example of cell stained with Giemsa dye with dicentric chromosome.

Our Calibration Curve Aberration per cell DOSE [Gy

Special thanks to: �Marta Deperas-Kamińska �Polina Kutsalo �Ekaterina Zaytseva

Thank you for your attention!