Regional Breeding for Wheat Stripe Rust Resistance in
Regional Breeding for Wheat Stripe Rust Resistance in the Eastern United States David Marshall Plant Science Research Unit Raleigh, NC
The U. S. has the most costeffective and plentiful food production and supply system in the world.
Wheat Stripe Rust
Stripe Rust Epidemic Years and the States with Greater than 1% Yield Loss from 1976 to 2004. % Loss Epidemic Year
Objectives: ØIdentify stripe rust resistance/susceptibility in Eastern U. S. wheat cultivars, elite lines, and other germplasm. ØIdentify the gene or genes involved resistance. ØIntrogress stripe rust resistance into elite lines having regional/local adaptation. ØDistribute populations and fixed lines to cooperators.
Protocol: ØEstablished the Uniform Eastern Wheat Stripe Rust Nursery. In 2005 -06, had up to 20 entries from State breeding programs in FL, GA, NC, VA, MD, NY, MI, MO, IL, IN, KY, AR, and LA; private breeding programs – Westbred, Agri. Pro-Coker, and Genesis; and USDA/ARS. ØSeedling screening in Raleigh, NC. ØField screening in Plains and Griffin, GA; Kinston, NC; Baton Rouge and Winnsboro, LA; Fayetteville, AR; San Antonio, TX, Pullman and Mt. Vernon, WA.
Designation Pedigree Contributor IT % Source Mean TERRAL LA 841 GA 8665 -D 4/GA 85238 -C 3 Harrison Louisiana 0. 00 GA 9615674 A 35 Jackson/2*881130 Johnson Georgia 0. 00 GA 975315 A 37 891138/Kinsco//PIO 2628 Johnson Georgia 0. 00 LA 99042 E 62 -B Ducula 1(H 2 O)/Mason, F 1//PIO 26 R 61 Harrison Louisiana 0. 75 0. 03 LA 96140 BU A 70 -2 VA 88 -52 -69/TX 89 D 6435//C 9134, F 1/3/Mason Harrison Louisiana 0. 75 0. 03 GA 9512314 E 26 881130/C 9134 Johnson Georgia 0. 25 0. 03 NC 03 -7222 L 910568/NCV 93 -1007//LA 854111 Murphy North Carolina 0. 25 0. 03 MO 050146 MO 94 -046/Pioneer 2552 Mc. Kendry Missouri 0. 25 0. 03 IL 87 -2834 -1 Tyler / Howell Kolb Illinois 0. 75 0. 03 992133 A 2 -1 -2 Patterson/F 201 R//INW 0101/4/Patton/3/Clark*4/Ning 7840//Foster Ohm Indiana 0. 25 0. 03
Identified 102 lines with resistance better than the "resistant" check (Pioneer 26 R 61). Infection types less than '3' and severities less than 18%. Identified adult-plant resistance in at least 8 lines: LA 95171 CA 58 -3 -2 -C CK 9134/PIO 2684, F 1//90239 A 29 -1 -X(CL 850643/PIO 2548//C 9877) 97 C-0554 -01 VA 94 -54 -549/Roane//Kristy B 010973 L 880085/XW 502 Coker 9511 L 881060/L 880436 IL 87 -2834 -1 Tyler / Howell IL 02 -5030 P 88288 C 1 -1 -6 -1 -2 -8 / 96 -6472 IL 03 -18438 97 -3574 / 95 -4162 INW 0412 Huapei 57 -2/Patterson
Several lines had combined stripe rust, leaf rust and powdery mildew resistance. FL 91226 A-X 4 Star'S'(IBW 179)/C 9105//F 302/P 2550, F 5 IDEO FL 98031 -D 15 -E 4 8952 -B-7/CM 98322 -0 M-2 Y, F 1//P 26 R 61 LA 95171 CA 58 -3 -2 -C CK 9134/PIO 2684, F 1//90239 A 29 -1 -X(CL 850643/PIO 2548//C 9877) LA 98094 -BUB-58 -5 90185 B 24 -4 -3/87167 D 8 -2 GA 96229 -3 A 41 881130/*2 881582 GA 961567 -4 A 35 Jackson/2*881130 GA 981621 -5 E 34 AGS 2485/PIO 26 R 61 GA 98249 G 1 -5 E 56 881130/ 2*901146 GA 011450 DH 2 -5 A 45 USG 3592/PIO 26 R 61 ARS 03 -3849 TX 85 -264*2/TTCC 512 (T. cylindricum) (=WX 02 ARS 149 -8) VA 01 W-205 PION 2684/93 -54 -185 (WLR/3/MSY*3/BALKAN//SAL)// POCAHONTAS, F 11 B 990133 L 890145/LX 8728 D INW 0412 Huapei 57 -2/Patterson 99840 C 4 -8 -4 961331/INW 0102//F 201 R/Foster E 01 -1053 U 90 -1 A//ZX 90 -2 C 1/P 2580
Regional Breeding scheme for introducing resistant sources: A. Backcross-Bulk (minor genes) 1. Several resistant sources x Adapted type 2. Backcross to Adapted type 3. Select in BC 1 through F 3 for low terminal severities B. Accelerated Population Development for major genes 1. Resistant source x Adapted type (crosses made in February) 2. F 1 planted in NZ in May, F 2 in NC in Nov, F 3 in NZ in June, F 4 in in Nov.
- Slides: 10