Redefining DNA Microarrays Tiffany Mc Aninch Advised by

Redefining DNA Microarrays Tiffany Mc. Aninch Advised by: Dr. Frederick Haselton, Ph. D Mark Mc. Quain, 2 BPh. D

Purpose Detect gene expression n Understand body and disorders n DNA -> m. RNA -> protein n

Northern Blotting

Microarrays Same info. , but hundreds at a time n Whole genetic distribution n

Microarray Procedure Target genes prepared and hyb. onto slides n Culture cells n Isolate m. RNA n Prepare labeled c. DNA n 2 c. DNAs competitively hybridized n Analyzed by fluorescent signature n

Microarray Schematic

Arrayer n $50, 000

Goals Design system to do same thing n Make quicker n Cheaper n Easier n

The Bead

Flow Cytometer PMT Dichroic Filters PMT Flow Manifold PMT Bandpass Filters SSC Laser FSC Cells

Comparison n Microbeads – – – – Easier to store Do chemically Hold patent! Less Reagents Speed Flow Cytometer 2 million signatures n Microarrays – Don’t need Raman tag – No flow cyt. for Raman – 50, 000 signatures

Work Completed Determined right beads and ordered n Learned Raman n Analyzed Raman of test beads and 2 ordered n Got primer for biotinylated targets and did PCR (polymerase chain reaction) n Calculated number of binding sites n

Work to do Hybridize fluorescent target DNA to beads n Hybridize without flur. and meausure Raman n m. RNA -> c. DNA n Flurescently label and hybridize to target DNA n Attach Raman tags n

Thanks!! Professor Haselton n Mark Mc. Quain n Professor Mahadevan-Jansen n Chad Lieber n Todd Monroe n