Recombinant DNA Technology Bacteria Yeast Virus Plasmids What

Recombinant DNA Technology Bacteria, Yeast, & Virus Plasmids

What is a Plasmid? • Circular DNA found in bacteria, viruses, and yeast

Recombinant Plasmids • Plasmids that have foreign DNA inserted into them in a process called Recombinant DNA Technology

Methods of Recombinant Technology • Transformation – Plasmid and foreign DNA are cleaved by restriction enzymes – Uses DNA ligase to insert DNA into vector – Vector inserted into host cell (usually E. coli)

Methods of Recombinant Technology • Non-Bacterial Transformation – Similar to transformation except E. coli/bacteria is not used as a host cell – DNA is injected directly into the nucleus of the cell being transformed OR – The host cells are bombarded with high velocity microprojectiles such as gold or tungsten coated with DNA

Methods of Recombinant Technology • Phage Introduction – Process of transfection (equivalent to transformation) except a phage is used instead of bacteria – Lambda or M 13 phages are used to produce phage plaques which contain recombinants

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