Recombinant DNA Technology Bacteria Yeast Virus Plasmids What
Recombinant DNA Technology Bacteria, Yeast, & Virus Plasmids
What is a Plasmid? • Circular DNA found in bacteria, viruses, and yeast
Recombinant Plasmids • Plasmids that have foreign DNA inserted into them in a process called Recombinant DNA Technology
Methods of Recombinant Technology • Transformation – Plasmid and foreign DNA are cleaved by restriction enzymes – Uses DNA ligase to insert DNA into vector – Vector inserted into host cell (usually E. coli)
Methods of Recombinant Technology • Non-Bacterial Transformation – Similar to transformation except E. coli/bacteria is not used as a host cell – DNA is injected directly into the nucleus of the cell being transformed OR – The host cells are bombarded with high velocity microprojectiles such as gold or tungsten coated with DNA
Methods of Recombinant Technology • Phage Introduction – Process of transfection (equivalent to transformation) except a phage is used instead of bacteria – Lambda or M 13 phages are used to produce phage plaques which contain recombinants
Antibiotic Resistance The Evolutionary Arms Race http: //www. youtube. com/watch? v=9 Cuhq. Qz. BACQ (Ep 1) http: //www. youtube. com/watch? v=2 L 82 V 6 VPJk. Q (Ep 2) http: //www. youtube. com/watch? v=D 0_FTJnhz. XA (Ep 3) http: //www. youtube. com/watch? v=of. L 52 t. Whac. Y (Ep 4) http: //www. youtube. com/watch? v=Chmb 2 g 7 at. Z 4 (Ep 5) http: //www. youtube. com/watch? v=J-VOvide. RZA (Ep 6)
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