RECIPE Mnchen Progress meeting 17 20052005 UK progress

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RECIPE München Progress meeting 17 -20/05/2005 UK progress Rebekka Artz Stephen Chapman Colin Campbell

RECIPE München Progress meeting 17 -20/05/2005 UK progress Rebekka Artz Stephen Chapman Colin Campbell Craigiebuckler, Aberdeen, AB 15 8 QH, UK

I. WP 01 UK Field site data • Middlemuir Moss, between Strichen and New

I. WP 01 UK Field site data • Middlemuir Moss, between Strichen and New Pitsligo, NE Scotland • Data collection finished, modelling in progress – meeting with Mika and Harri (20 -22/04) to kick this off

Dark CO 2 production Bare peat, total respiration models (r 2 = 0. 6

Dark CO 2 production Bare peat, total respiration models (r 2 = 0. 6 (WT), 0. 82 (T @ -5 cm)

CO 2 fluxes 2 – PGCO 2 E. angustifolium + S. auric. collar, r

CO 2 fluxes 2 – PGCO 2 E. angustifolium + S. auric. collar, r 2 = 0. 72

Modelling problems • Not enough data to model per collar, trying to overcome this

Modelling problems • Not enough data to model per collar, trying to overcome this by pooling data from replicate collars (Mika found that this doesn’t always work well…) • Rtot modelling produces very low r 2 values – a lot of data had to be excluded as either missed dark measurements or negative dark fluxes • PGCO 2 modelling ok, but lack of LAI data forces use of other seasonality index, both air and -5 cm temps seem to work ok

II. WP 2 Progress • Problems with negative dark fluxes (i. e. CO 2

II. WP 2 Progress • Problems with negative dark fluxes (i. e. CO 2 uptake) since july 2004 – these appear to have been at least partially caused by our large chambers and an overly sensitive IRGA system (when checking data all these fluxes had low r 2 values, i. e <0. 8) • Data collection since new (much smaller) chambers/PPsystems IRGA (february 2005) much more stable and no more uptake in the dark! • Methane analyses have been hampered by GC breakdown at the start of this year and lack of tech support (back log/loss of 2 or 3 sampling points this year). • Data analysis lagging behind…. will try to keep running the remaining replicates beyond june harvest!

Further work: • 1 st harvest starting 6. 06 (pending decision) • Clare Trinder

Further work: • 1 st harvest starting 6. 06 (pending decision) • Clare Trinder is using the field site for litter bag experiments, 5 species (E. ang/vag; S. subnitens? ; Calluna vulgaris, spruce) and will look at decomposition rates and microbial diversity in the litter bags. Litter quantities in the field estimates by traps around small plants • Also being set up by Clare: decomposition experiments with added live plants, also involving 14 C labelling of the plants to chase carbon

Fungal community structure as Indicators of change? • fungal ITS clone library from SC

Fungal community structure as Indicators of change? • fungal ITS clone library from SC set , 150 clones sequenced. Most sequences showed < 70 % homology with any sequence in Genbank. Paper is now in prep. FI EARLY LATE FR CH

III. Fungal community structure analysis

III. Fungal community structure analysis

Horizon Nearest neighbour to clones in clone list (% homology gapped) A 31 1

Horizon Nearest neighbour to clones in clone list (% homology gapped) A 31 1 Cryptosporiopsis / Cladosporium (86) 3 Saccharomyces ? (57) 4 + 8 +9? Exidiopsis ? (74) 5 Penicillium sp (79) 10 – low overlap < 150 bp 11 Microdochium or Phoma sp (65) 12 Saccharomyces ? (60) 13 Cortinarius sp ? (62) * A 32 37 Penicillium sp (78) 39 Saccharomyces ? (57) 40 Cladosporium / Cryptosporiopsis (83) 45 Capronia (92) (low) Fusarium (80) 48 Lodderomyces ? (63) * A 41 70 Fusarium / Sclerotinia (80) 82 Phialocephala sp (88) * 85 Sclerotinia (67) * 92 Phialocephala / Cadophora (89) * 99 Sclerotinia (71) * A 42 105 Fusarium / Gibberella (76) A 61 848 Tomentella or rel (89) A 62 153 Fellomyces? (65) 12 Saccharomyces ? (60) 851 Cordyceps bassiana (99) 6 Dioszegia – hymen. Yeast? (69) 108 Phialophora/ Cadophora (86) 113 Cadophora / Phialophora sp (80) * 116 Phialophora / Cadophora (90) ** 121 Kockovallia ? (67) 851 Cordyceps bassiana (99) 858 Gyoerfyella rotula (95) PD PD 008 Malassezia restricta (98) PD PD PD 164 Cortinarius sp (90) 174 Fusarium ? (83) * 910 Mortierella /Verticillium ? (67) * 915 Phialophora / Cadophora (80) 919 Geomyces pannorum (98) A 82 184 Stereum ? – low overlap 450 (66) * 187 Capronia / Dermatocarpon (86) ** 188 Scleroconidioma? (88) * 191 low overlap 400 (60) v long seq 004 Trichosporon (98) B 32 206 Dermea ? (89) * 217 Mycorrhizal sp. (92) 219 Taphrina / Lalaria low overlap (85) * 220 Scuttellospora/ Candida (66) * 222 Cryptosporidiopsis /Pezicula ? (84) B 33 232 Saccharomyces (61) 237 Saccharomyces ? (60) * 238 Hypholoma (95) * 239 Kluyveromyces – low overlap (66) * 240 Umbilicaria (81) B 41 267 Ascocoryne ? DSE (86) * 270 Saccharomyces – low overlap (67) ** 275 Hymenoscyphus (70) 277 Peniophora / Trametes (75) 284 various (Ians clones) (80) B 42 297 Phialaphora / cephala (81) 304 Sebacina endomycorrhiza (83) 308 Sebacina endomycorrhiza (88) ** 312 Sebacina endomycorrhiza (88) 315 Sebacina endomycorrhiza (89) B 61 321 Saccharomyces – low overlap (90) * 322 Phialaphora /Phialacephala (86) 324 various Russula Puccinia etc (60 -70) 325 Cryptococcus (99) 330 various Russula Puccinia etc (60 -70) B 62 942 Phialocephala (88) 341 Ascocoryne (86) * 946 – failed 945 Trechispora ? (76) ** 950 Sclerotinia sclerotium (100) B 81 351 Leptodontidium /DSE (85) * 352 Fusarium oxysporum (97) PD 354 Ascocoryne (84) 358 Leontopodium ? (78) B 82 977 Phialocephala? – low overlap (68) 983 uncultured clones (Ian’s) (99) 984 Cladosporium ? – short sequence (91) A 81 312 Sebacina endomycorrhiza (88)

C 31 377 Sclerotinia ? * 396 Sebacina mycorr. (90) 399 Kluyeromyces (62) 400

C 31 377 Sclerotinia ? * 396 Sebacina mycorr. (90) 399 Kluyeromyces (62) 400 Cadophora /Cephalosporium (83) * 402 Mortierella /Verticillium ? (83) C 32 407 clones Ian (81) + Hygrocybe (58) 409 Sebacina mycor. (63) clones Ian (77) 415 Archaeospora (71) 424 Demea/ Guignardia/ Pezicula (78) 422 clone ectomyc. (8597) * C 32/33 427+ 429 low overlap 436 Gymnopilus (61) 438 Mycosphaerella / Cladosporium (98) 450 Mycocalicium / Mycosphaerella (87) 451 Cordyceps/ Cryptosporiopsis ? (93) C 41 455 Venturia/ (pseudo)cladosporium (90) 460 Alatospora /Neofabraea ? (70) 463 Sebacina clone (82)/ Polyporus ? (69) 464 Fusarium ? (78) 466 Sebacina clone (81)/ Polyporus ? (69) C 42 473 myc isol (99)/ Gyorffyella (95) * 476 Hymenoscyphus (75) 488 myc isol (99)/ Gyorffyella (95) 491 Archaeospora/ Phoma ? (low) (69) 493 Tricholoma / Cortinarius (67) C 61 505 Phialocephala ? (88) * 509 Alatospora /? (81) 510 Phialocephala ? (88) * 511 Russula ? (64) * 514 Cordyceps bassiana (99) C 62 522 Leptodontidium + clones (78) 526 Leptodontidium + clones (77) 531 Leptodontidium + clones (78) 534 Leptodontidium + clones (78) 542 Leptodontidium + clones (77) C 81 546 Cryptococcus/ Filobasidium (78) 552 Cryptococcus/ Filobasidium (78) 555 Cladosporium (86) ? 559 Cladosporium etc (85) ? 562 Malassezia globosa (98) C 82 566 Phialocephala/spora (86) * 567 Leptodontidium + clones (78) 572 Wilcoxina ? (82) 576 Marasmius / Gymnopus (99 -90) 581 Leptodontidium + clones (78) D 31 590 Saccharomyces – low overlap (62) * 595 Neofabraea etc ? (85) 602 Phialocephala /phora (75) 606 Pholiota/ Psylocybe (84) ** 615 Cladosporium/ Venturia (86) D 32 625 + 637 Mycena (92) 630 Phialophora/various mycorrh (86) 650 Leptosphaericea (90) 642 Hypocrea (90) 644 Cordyceps/ Tolypocladium (89) D 41 650 Leptosphaeria (90) 655 Cryptosporidiopsis ? (81) 664 Cordyceps /Tolypocladium (90) 678 Galerina / Gymnophilus (96) 673 Chaetosphaeria (89) D 42 691 Kluyveromyces / Russula (low) (60) 693 Alatospora + clones (82) 697 Gigaspora ? (low) (71) 707 Alatospora + clones (86) * 711 clones only (84) D 61 720 Phialocephala /phora (81) + clones 723 clones (77) 724 Trametes / Mortierella (low) (67) * 733 Phialocephala /phora (87) + clones 744 Scutello/Gigasora (low) (67) D 62 753 Phialophora (87) + clones 756 Leptodontidium + clones (79) 759 Phialocephala (88) * 765 Gigaspora/ Tremella (low) (66) 772 Cercophora/ Lasiosphaeria ? (81) D 81 784 clones/ Dactylella ? (73) 797 Fusarium oxysporum (98) 799 clone (77) + Cordyceps (low) (91) 803 various (low) (74) 812 Phialophora + clones (89 -96) D 82 815 Russula ? (58) * 820 clones Asco (72) * 822 Phialocephala + clones (87) 827 Leptodontiodium + clones (78) 836 Bullera ? (65) 476 Hymenoscyphus (75) 562 Malassezia globosa (98) 625 + 637 Mycena (92)

Exidiopsis etc/ Cryptococcus etc. Includes Polyporales & Thelephorales Hymenoscyphus Cordyceps, etc, & Peziculales Cephalosporium,

Exidiopsis etc/ Cryptococcus etc. Includes Polyporales & Thelephorales Hymenoscyphus Cordyceps, etc, & Peziculales Cephalosporium, Fusarium etc. Chaeto/Lasiosphaera & Cercophora

IV. Community level physiological profiling • paper submitted to Environmental Microbiology detailing the adapted

IV. Community level physiological profiling • paper submitted to Environmental Microbiology detailing the adapted peat Micro. Resp method and validation data • validated the method in three experiments: 1) optimum incubation time 2) sample moisture content and 3) sample ageing due to sample transport and storage • needs to be re-submitted to EM (hopefully mid june)

CVA scores SD (n = 3) of Moss (filled squares), Decaying moss (open squares),

CVA scores SD (n = 3) of Moss (filled squares), Decaying moss (open squares), Newly formed peat (filled triangles) and Old catotelm peat (open triangles) after 4 h (plot A), 20 h (Plot B), 48 h (Plot C) and 120 h (Plot D) of incubation.

Effect of adjustment of moisture content on CLPP-SIP patterns. Peat samples were adjusted to

Effect of adjustment of moisture content on CLPP-SIP patterns. Peat samples were adjusted to 80 (filled squares), 85 (open squares), 90 (filled triangles) and 95 % GWC (open triangles). Data show the mean CV scores SD (n = 3)

Samples of different degrees of decomposition were stored at 4ºC for 7 d (filled

Samples of different degrees of decomposition were stored at 4ºC for 7 d (filled squares), 28 d (open squares), 49 d (filled triangles), 70 d (open triangles) and 103 d (open diamonds). Data show the mean CV scores SD (n = 3)

V. FTIR and a request… • Now in the process of working up the

V. FTIR and a request… • Now in the process of working up the WP 1 FTIR data. We found some interesting patterns in the carbohydrate signal region that may tie up with the vegetation structure – our FTIR specialist would be very interested in some clean homocellulose or other (sugar? ) fractions for FTIR…. • another region indicating free acids ties up closely with p. H values in the SC set, more environmental data are needed to check this for all WP 1 data • a combination of the free acid, carbohydrate and ‘lignin’-marker regions may be useful as an index for humification – still in progress