RABIES VIRUS Whats this Morphology and structure Replication

RABIES VIRUS

What’s this?

• Morphology and structure • Replication • Pathogenesis • Clinical symptoms • Diagnosis • Prevention and Treatment

Morphology • bullet-shaped; • 45 -100 nm in diameter; 100 -430 nm long.

structure • Core • • Capsid P-protein Envelope • • • -ss. RNA L-protein (large protein) ---RNA dependent RNA polymerase N-protein ---protect RNA from hydrolysis Bilipid layer M-protein (matrix protein) G-protein (glucoprotein) ---spike ---bind to the receptor of the host cell RNP

• Rabies is an RNA virus. The genome encodes 5 proteins designated as N, P, M, G, and L. The order and relative size of the genes in the genome are shown in the figure below. The arrangement of these proteins and the RNA genome determine the structure of the rabies virus. •

Replication • Adsorption • Penetration • Uncoating • Biosynthesis • Assembly and release

biosynthesis • • • -ss. RNA polymerase +ss. RNA -ss. RNA protein virus

Pathogenesis • CNS afferent fibers nerve endings Neuromuscular junction muscles Virus efferent fibers salivary gland organs and tissues

Clinical symptoms • Early stage (1 -3 days) • fever(37. 5 -38) • vomit • wound: pain, red, itching • Exciting stage(1 -3 days) • fever(39 -40) • hydrophobia: pharyngeal muscle cramp, dyspnea • dehydration • Paralytic stage(15 -20 hs) • nerve fiber paralysis • respiratory and circulatory failure death

Diagnosis • • Pathogenic history Isolating the dog(7 -10 days) cerebral smear or slice immunofluorescent stain

Prevention and treatment • Kill wild dogs • Keep away from dogs • Clean the wound • Passive immunity • vaccine

Contributers • Bian Shu • Cheng Wei • Cong Ping • Hu Chen • Mu Xueru • Li Yuntao • Shao Cong • Li Daowei • Zhang Jingjing • Wang Shengxi • Zhu Wenying