Quattro Mix Single Use Mixing System For Single

Quattro. Mix™ Single Use Mixing System For Single Use Bag Integration work In cooperation with:

Quattro. Mix Principles • Application: Single Use Bag Exocirculation™ Via pump Theoretical fluid stream • Random Vector™ No Vortexing 16 outlet nozzles Design Connected with pump in- and outlet

Performance test 500 L 120% 100% % of final conductivity Solid/liquid mixing performance: Addition of Na. Cl to 500 L Water 80% 60% 40% 20% 0% 0 1 2 3 Time [min] 4 5 2 g/l; Completely dissolved after 5 min 6 80% 60% 40% 20% 0% 0 1 2 3 4 5 6 7 Time [min] 8 9 10 11 4 g/l Completely dissolved after 10 min 500 L bag with two Quattro. Mixers, in parallel connected to one QF 1200 SU.

Applications Typical application in biopharmaceutical processes Buffer preparation Salts Carbohydrates Purified Water Acids/ Bases Nutrients Media preparation Virus inactivation And others SU Bag with Mixer Sterile Filtration Upstream or Downstream Process

Quattro. Mix Enables: • Quattroflow Pump becomes a multi purpose device, because – Recirculation of liquid is used for mixing effect – Can be used for filling and emptying of the bag – Can be used for post-mixing processes, like sterile filtration, virus filtration, flow through chromatography, etc. • Recirculation loop allows for additional features like – Integration of in-line sensors (p. H, conductivity, temperature, UV) – Usage of heat exchanger for cooling/heating of media TIR Heat exchanger Filter Feedstock Filtrated Product

Comparison 200 L 120% 100% % of final conductivity] % of final conductivity Solid/liquid mixing performance: Addition of 500 g Na. Cl to 200 L Water Result: Better performance of QF mixer 80% 60% 40% 20% 0% 0 100 200 Time [sec] 300 Completely dissolved after 4 min 400 80% 60% 40% 20% 0% 0 100 200 Time [sec] 300 Still undissolved Na. Cl after 18 min Jet. Mixer 400

Performance test Liquid/liquid mixing performance: adding 100 g predesolved Na. Cl to 200 L Water Result: Comparable mixing performance: 140% 120% % of final conductivity 120% 100% 80% 60% 40% 20% 0% 0% 0 20 40 60 Time [sec] 80 100 0 20 Jet. Mixer 40 60 Time[sec] 80 100

Comparison 200 L Quattro. Mix Jet. Mixer

Available For: • Application development work • Mixing problem resolution Bag Sizes: • 100 L, 200 L with single mixer • 500 L with dual mixer Bag Configuration/porting • On request to HPNE From: HPNE