Quantitative And Qualitative Analysis In Electron Microscopy Prof

Quantitative And Qualitative Analysis In Electron Microscopy Prof. Dr. Ömer ANDAÇ

DERS İÇERİĞİ • 1 Electromagnetic spectrum, Interaction of electromagnetic radiation with matter • Elektron, De Broglie dalga boyu, Büyütme oranı dalga boyu ilişkisi, Optik Mikroskop, • 2

Electron–specimen interaction Instrumentation, Electron Emitters, Electron Lenses Specimen Preparation in Materials Science Procedure of sample preparation of a biological specimen Secondary electron imaging of topographic features Backscattered electron imaging of chemical phase difference Qualitative and quantitative elemental analysis with EDS Qualitative and quantitative elemental analysis with WDS Mid-Term Exam X-ray elemental reference standards for energy dispersive or wavelength dispersive X-ray microanalysis X-ray imaging: elemental line scans and maps SEM - vs - STEM (TEM) EBSD SEM Cathode-Luminescent Studies

ELECTROMAGNETIC SPECTRUM • Radiowave • Microwave • IR • UV • Visible • X-ray • Gamma

Radiation and the Human Body INTERACTION OF ELECTROMAGNETIC RADIATION WITH HUMAN http: //hyperphysics. phy-astr. gsu. edu/hbase/mod 3. html

INTERACTION OF ELECTROMAGNETIC RADIATION WITH MATTER http: //hyperphysics. phy-astr. gsu. edu/hbase/mod 3. html

INTERACTION OF ELECTROMAGNETIC RADIATION WITH MATTER Spectroscopy: Interaction of light and matter All imaging systems map some spectroscopic property of the imaged object onto a detector. • Nuclear Magnetic Resonance (NMR) spectroscopy • Electron paramagnetic resonance (EPR) or electron spin resonance (ESR) spectroscopy • Rotational Spectra : Microwave Spectroscopy • Infrared spectroscopy (IR spectroscopy) • Ultraviolet-Visible (UV-Vis) Spectroscopy • X-ray fluorescence (XRF)spectroscopy • X-ray photoelectron spectroscopy (XPS)

MICROSCOPY • A microscope is an instrument that magnifies objects otherwise too small to be seen, producing an image in which the object appears larger Traditional Microscopes • Optical Microscope; • Scanning Electron Microscope (SEM); • Transmission Electron Microscope (TEM);

Optical Microscope The optical microscope, often referred to as the “light optical microscope, ” is a type of microscope that uses visible light and a system of lenses to magnify images of small samples The optical microscopy is a basic form of microscopic technology that depends on its analysis on the interaction between the light and matters

Magnification • The numerical aperture of a microscope objective is the measure of its ability to gather light and to resolve fine specimen detail while working at a fixed object (or specimen) distance. • Useful Magnification (total) = 500 to 1000 × NA (Objective)

Numerical Aperture (NA) = η • sin(α)(1) where α equals one-half of the objective's opening angle and η is the refractive index of the immersion medium used between the objective and the cover slip protecting the specimen (η = 1 for air; η = 1. 51 for oil or glass). Numerical Aperture (NA)

Resolution The resolution of an optical microscope is defined as the smallest distance between two points on a specimen that can still be distinguished as two separate entities. Resolution is directly related to the useful magnification of the microscope and the perception limit of specimen detail, though it is a somewhat subjective value in microscopy because at high magnification, an image may appear out of focus but still be resolved to the maximum ability of the objective and assisting optical components