Purification To obtain the viral particles in a
Purification To obtain the viral particles in a single form out of cell debris i. e. removal of all impurities.
Purification outline 1. Preparation of harvest. 2. Methods of purification. 3. Criteria of purity. 4. Maintenance of purified virus.
Preparation of harvest Bacteria Animal Plant Insect
Methods of purification 1. Filtration. 2. Centrifugation. 3. Adsorption. 4. Precipitation. 5. Solvent extraction. 6. Other methods.
Methods of purification 1. Filtration. 2. Centrifugation. 3. Adsorption. 4. Precipitation. 5. Solvent extraction. 6. Other methods.
Filtration : 1. Ultrafiltration 2. Gel filtration
Ultra-filtration
Gel filtration
Methods of purification 1. Filtration. 2. Centrifugation. 3. Adsorption. 4. Precipitation. 5. Solvent extraction. 6. Other methods.
centrifugation Differential Isopycnic Gradient Cushion
Methods of purification 1. Filtration. 2. Centrifugation. 3. Adsorption. 4. Precipitation. 5. Solvent extraction. 6. Other methods.
Adsorption : 1. Ion exchange 2. Heamadsroption
Ion exchange chromatography
Heamadsorption
Methods of purification 1. Filtration. 2. Centrifugation. 3. Adsorption. 4. Precipitation. 5. Solvent extraction. 6. Other methods.
Precipitation : 1. Chemical • Ammoniumsufate • PEG • Zinc acetae 2. Physical • Isoelectric piont (p. H change)
Methods of purification 1. Filtration. 2. Centrifugation. 3. Adsorption. 4. Precipitation. 5. Solvent extraction. 6. Other methods.
Methods of purification 1. Filtration. 2. Centrifugation. 3. Adsorption. 4. Precipitation. 5. Solvent extraction. 6. Other methods.
Other methods • • Electrophoresis. Serological methods. Enzymatic reactions. HPLC
Electrophoresis
HPLC
Criteria of purity Chemical purity Physical purity Biological purity
Criteria of purity E. M. Spectrophotometer serological Isoradiation Infectivity
Criteria of purity E. M. Spectrophotometer serological Isoradiation Infectivity
Marking
Criteria of purity E. M. Spectrophotometer serological Isoradiation Infectivity
Infectivity: 1. Local lesion assay 2. Plaque assay
Virus preparation
Criteria of purity E. M. Spectrophotometer serological Isoradiation Infectivity
Criteria of purity E. M. Spectrophotometer serological Isoradiation Infectivity
4 - Maintenance: The half life of infectivity can be measured in seconds at 60 o. C; minutes at 37 o. C, hours at 20 o. C , days at 4 o. C and years at -70 o. C or lower, (Fenner et al, 1987) Lyophlization
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