Protein sequences alignment and protein modeling Protein sequences

Protein sequences alignment and protein modeling 唐世杰老師實驗室: 廖吉隆

Protein sequences alignment

• Proteins sharing a significant degree of sequences similarity and structural resemblance are said to be homologous. PNAS (2000, 97, 8762 -8769)

The amino acid sequences of human hemoglobin, Bos taurus and Mus musculus show a strong degree of homology.

• If same kind of proteins share homology, it can be revealed through alignment of their sequence is using powerful computer programs. 1. Vector. NTI 2. Genedoc • We can use protein sequence alignment to search unknown protein you interesting.

• Protein sequence databases provide enormous resources for sequence comparisons. 1. National Center for Biotechnology Information (NCBI) 2. Expert Protein Analysis System (Ex. PASy)

Protein modeling

• SWISS-MODEL is a fully automated protein structure homology-modeling server, accessible via the Ex. PASy web server. • The purpose of this server is to make Protein Modelling accessible to all biochemists and molecular biologists worldwide.

Alignment Unknown protein structure Modeling Similar protein sequence

(1) PI 3 K (2) P 21 (3) P 53 (4) GAPDH (5) NF-k. B (6) Caspase 8 (7) AMPK (8) Topoisomerase (9) DNA helicase (0) Hemoglobin