Prostate Transverse section Radical Prostatectomy Apex Bladder neck
Prostate: Transverse section
Radical Prostatectomy Apex Bladder neck
Radical Prostatectomy Base/ Bladder Neck Apex Posterior quadrants of alternate slices have been removed and frozen after inking the surface
Lateral aspect Seminal Vesicle 1 right Schematic of how samples of tumor are obtained from a radical prostatectomy specimen, without compromising sampling or diagnostic pathology data Permanent section left 2 3 4 5 6 7 5 R 5 R 5 5 L 5 L Fresh, non-frozen tissue for flow sorting Frozen section right Unfixed tissue for RNA & DNA left Frozen section 4 R 4 L 4 L Permanent section right left permanent section 3 R 3 3 R 5 L = Prostate carcinoma 3 L
Human prostate (lateral view) Seminal Vesicle Obtaining samples of non-tumor tissue from a radical prostatectomy specimen. Permanent section 1 2 3 4 5 6 7 right left Fixed tissue: In-situ hybridization 5 R 5 R 5 This block is snap frozen (isopentane in liquid N 2), right within < 20 minutes of devitalization 4 5 L 5 L Fresh, non-frozen tissue: cell sorting Frozen tissue: Immunostains Laser capture left 4 R 4 R 4 L 4 L Frozen section Permanent section right left Permanent section 3 R 3 3 R 5 L = Prostate carcinoma 3 L
Prostate (transverse section)
Normal prostate parenchyma (high magnification) Basal epithelial cells Secretory (luminal) epithelial cells Fibromuscular stromal cells
Normal prostate parenchyma (low magnification) fibromuscular stroma normal glands lymphocytes
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