Project No SI03BFPP176012 FKKT Ljubljana 2003 2005 HANDSON

Project No: SI/03/B/F/PP-176012 © FKKT, Ljubljana, 2003 -2005 HANDS-ON APPROACH TO CHROMATOGRAPHY Nataša Gros 1 Margareta Vrtačnik 2, M. Filomena Camões 3 1 Faculty of Chemistry and Chemical Technology, 2 Faculty of Natural Sciences and Engineering, University of Ljubljana, Slovenia 3 University of Lisbon, Portugal

LEGEND Video clip Experiment Navigation – next Navigation – back to modules Next slide

1. Introduction to chromatography 2. Gas chromatography 3. Liquid chromatography 4. Chromatography –fields of application

Examples of slides

A simplified gas chromatograph Flow regulator ¸? 2 Record of measurement values display Cylinder with carrier gas Detector Column with stationary phase Injector

Chromatogram Chromatographic peak of the component eluted first Chromatographic peak of the component eluted second R E S P O N S E Graphic presentation of measurement values vs time

A simplified liquid chromatograph

Chromatographic system with vacuum water-jet pump Pump Container with eluent display Sample injection port ¸? 2 Record of measurement values Detector Column with stationary phase

“ Separating the dyes from mint aroma Video: click on the icon

“ Separation of the dyes from mint aroma Green component blue component

Acknowledgements This Project has been carried out with the support of the European Community. The content of this project does not necessarily reflect the position of the European Community, or National Agency. For this reason neither the National Agency nor the European Community take any responsibility regarding the content of the report.