Produkn biologie rostlin Metody fluorescence chlorofylu Podzim 2012

Produkční biologie rostlin Metody fluorescence chlorofylu Podzim 2012 Fotoinhibice fotosyntézy fluorometrické metody stanovení M. Barták

http: //www. esf esf. . edu//efb efb//schulz/Limnology/phytoecol 1. html

http: //www. jeffs 251 lab. com/category /lab 4/ http: //www. jeffs 251 lab. com/category/lab 4/

Basic de-excitation mechanisms of Chl a Photosynth. process (linear transport e- ) Chl a* Thermal dissipation Regulation between PSII and PSI Chlorophyll fluorescence

Photosynthetic pathway Qa 3(P 680+ Pheo-) 1(P 680+ Triplet Recomb. 3 3 Reversible transport Pheo-) Singlet Recombin. P 680* 1 B carotene* P 680 heat O 2* 3 B carotene* 3 Chla* heat P 680* Fluorescence Chla* Antennae q. Charge separation

Fv/Fm : potential yield of photochemical processes in photosystem II daily course of Fv/Fm indicates light-dependent adjustment of photosynthetic processes (lower effectivity ) http: //www. clr. . pdx. edu/projects/lakes/waldo. php

Slow Chl fluorescence kinetics with quenching analysis q. P = (Fm’- F) / (Fm’-Fo’) NPQ = (Fm - Fm’) / (Fm’) q. N = q. E + q. T + q. I q. E (energy-dependent quenching) Fm’-= Fms

Control – before stress treatment Just after stress treatment Partial recovery After 60 min. in dark http: //www. biol. s. utokyo. ac. jp/ jp/users/seitaipl/personal/terashima/tera shima_e. html