Product specification Software specification Main exclusive features Overview

• Product specification • Software specification • Main - exclusive features • • Overview Parameters Reagents & Controls Rack loader & sample principles CDR Measurement principles Customer maintenance Environmental protection

Overview Compact bench top system No external computer is requested Touch screen Reagent Compartment Rack Loader 10 racks of 10 tubes Processed Tubes Stat Sample Bay

Parameters CBC Mode (12 Parameters) WBC, RBC, HGB, HCT, MCV, MCHC, RDW, PLT, MPV, PCT, PDW 5 DIFF Mode ( 26 Parameters) WBC, RBC, HGB, HCT, MCV, MCHC, RDW, PLT, MPV, PCT, PDW LYM, MON, NEU, EOS, BASO (% and #) ALY (Atypical Lymphocytes) (% and #) LIC (Large Immature Cells) (% and #) 80 samples per hour Manual or automatic CBC or DIF (random access)

Reagents & Controls 5 reagents ABX ABX + ABX Cleaner Biolyse Basolyse II Eosinofix Diluent Onboard reagents Bar-coded Waste level sensor Control bloods : Bar-coded Target values uploaded by floppy disk

Rack loader Sampler capacity : • 10 racks of 10 samples XL walk-away autonomy : 1 hour 15 mn

Sampling Automatic mode with end over end mixing 30 µl (CBC) 53 µl (CBC+DIFF) Manual (open or close) mode Internal and external barcode reader

CDR - Purpose CDR = Customized Dilution Ratio Purpose : Pushing the limits of linearity without blood manipulation and in a very user friendly way The CDR eliminates manual dilution error and secure the result !

CDR - Operation The end-user can use the CDR in 2 modes : 1 - Manual selection of the dilution ratio The end-user can program 3 dilution ratios:

CDR - Operations 2 - Automatic selection of dilution ratio CDR are programmed to rerun samples for every “DIL” flag with predefined dilution ratio

Measurement principles RBC/PLT - Impedance HGB - Cyanmethemoglobin or cyanide free WBC - Impedance & optical BASO - Impedance & specific lyse

Measurement principles 1. Cytochemistry Lyses red blood cells Stains nuclei and cytoplasmic granules of leukocytes Fixes the leukocytes in their original state (48 hours post-draw stability) 2. Double hydrodynamic focusing (DHSS) Flow-cytometer with hydrodynamic focusing before and after the aperture 3. Impedance method Cell volume (“X” axis) 4. Light absorbance Analysis of the internal structure (“Y” axis”)

Measurement principles The determination of the 5 per differential uses : X -> Cellular volume Y -> Intensity of staining Eosinophils Large Immature Cells Neutrophils Lymphocytes Monocytes Atypical Lymphocytes

Flags and alarms General flags • “l” and “h” “L” and “H” “x” “!” (normal limit) (panic limit) (reject) (suspicion) • Pathologic messages RBC and PLT curves

Flags and alarms 5 per differential matrix 1 - Priority alarms ALY: (Atypical Lymphocytes) Atypical Lymphocytes Plasmocytes, hairy Cells LIC: (Large Immature Cells) Blasts, myelocytes Promyelocytes, Metamyelocytes

Flags and alarms 5 per differential matrix 2 - Separation alarms Nl: (Neutro-lympho) Hypo-segmented neutrophils Sezary cells, hairy cells Ne: (Neutro-eosino) Hyper-segmented neutrophils Hypo-granular eosinophils Mn: (Mono-neutro) Immature granulocytes, band cells

Flags and alarms 5 per differential matrix 3 - Zone alarms No: (Noise) Platelet aggregates, NRBCs Lysis resistant RBCs Ln: (Left-neutro) Cellular debris Ll, Ll 1: (Left-lympho) Platelet aggregates, NRBCs Rm: (Right-mono) Large monocytes, blasts Rn: (Right-neutro) Immature granulocytes

Customer maintenance Only one automatic shut-down per day Stepper motors replace the old compressor concept -> No noise, no maintenance A double needle replaces the sampling valve concept -> No clogging, no maintenance Liquid valves replace the old pinch valve concept -> No clogging, no maintenance

Environmental protection Liquid waste 32 ml / sample Solid waste 1, 83 g / sample

Environmental protection Power Requirements Power supply From 100 V to 240 V (50 HZ to 60 Hz) Power consumption Maximum 230 VA

• Product specification • Software specification • • • Main - exclusive features • • System specification Worklist Data processing Validation station

System Colour LCD touch screen Windows XP Query mode Random access (DIFF or CBC mode) Onboard validation-station Onboard user manuel Real-time management of patient’s report

System 9 logs for : Maintenance Calibration Controls Errors Data Handling Patient record Settings Blank cycles Connection

Worklist Manual entry or host connection Easy to set up Real time visualisation Maximum security Demographic data, department. . . Comment

Automatic validation based on: • Flags, • Alarms, • Limits, • Delta check

Building up a patient report (1) Patient report built by selective choice from: • Run • Re-run • Manual entry • Pathology level

• Product specification • Software specification • Main - exclusive features

Main features Qualification & quantification of ALY and LIC Automatic and immediate rerun Real manual open tube and manual close tube Real capillary mode (30 µl for CBC, 53µl for CBC+Diff) Integrated validation station Compact, simple, reliable (no shear valve, no compressor, only one PCB) Automatic shutdown cycle - No customer maintenance Small reagent consumption Low power consumption