Processed Amniotic Membrane Allografts Amniotic Membrane Allografts What

Processed Amniotic Membrane Allografts

Amniotic Membrane Allografts

What is Human Amniotic Membrane? • A unique, avascular membrane separating the mother from the fetus. • Provides an incubating environment promoting cellular differentiation. • Provides an immunological barrier to prevent “foreign body” rejection. Photo: Courtesy of Juan Batlle, M. D.

Anatomical Profile: AM

Anatomy of the Ocular Surface (1) Conjunctival Epithelium (2) Corneal Epithelium (6) Limbus – Stem Cells

History of AM in Ophthalmology • • De Rotth. conjunctival defects (1940). Lavery. lime burn of conjunctiva and cornea (1946). Sorsby et al. caustic soda burns (1947). ALLOTRANSPLANTAT [late 80’s USSR->Venezuela, DR] • Batlle and Perdomo. Conjunctival substitute with placental allotransplant. Scientific Poster 25. American Academy of Ophthalmology meeting. Chicago, IL USA. October 1993. • Kim and Tseng. Transplantation of preserved human amniotic membrane for surface reconstruction in severely damaged rabbit corneas. Cornea 14: 473 -84, 1995. [

Surgical Indications: AM • • Pterygium Excision Corneal Ulcerations/Perforations Chemical/Thermal Burns Bullous Keratopathy Ocular Dermoids/Tumors Fornix Reconstruction/Symblepharon Stem Cell Transplants

Pterygium • A mutated growth on the surface of the eye • Requires surgical excision and placement of graft

Corneal Ulcerations/Perforations • A break or defect in corneal epithelium • Often secondary to other systemic diseases.

Chemical/Thermal Burns • Chemical burns often caused by alkali • Potentially devastating trauma to surface of the eye • Limbal graft possibly indicated

Bullous Keratopathy • Edema of the corneal endothelium • Very common and usually affects individuals over 50 years of age.

Dermoid/Tumor Removal • Benign congenital tumors containing foreign tissue • Commonly found at the limbus

Fornix Reconstruction • Fibrous tract that connects bulbar conj to conj of eyelid • Secondary to other acquired or traumatic conditions • Required reconstruction of ocular surface and eyelids

Photo: Courtesy of Juan Batlle, M. D. Surgical Techniques: AMT/Pterygium

Ambio. Dry 2 Overview • • Tested & Safe Dehydrated Terminally Sterilized Strict, qualitycontrolled protocols • Device-like quality

Ambio. Dry: Safe & Viable • Intact epithelial cell layer • Intact dense connective, basement membrane • Presence of loose fibroblast network

Ambio. Dry: Logistical Features • Storage: Room-temp • No freezer required • No dry ice shipments • Simple prep: No soaks or rinses • IOP Customer Service: 24 Hrs/7 Days

Ambio. Dry: Surgical Advantages • Substrate-free • Dry-state handling & trimming • Visual orientation identification • No tears or buttonholes • Device-like tissue quality

Ambio. Dry Configurations • 1 x 2 cm • 2 x 3 cm • 4 x 4 cm

Reimbursement Codes Supply Code: V 2790 CPT Code: 65780 Ocular surface reconstruction; amniotic membrane transplantation

Ambio. Dry & Tisseel The sutureless approach to eye surgery. Fibrin (biological) adhesive Eliminates sutures Reduces surgical time Improved patient care Better healing

Ambio. Dry & Tisseel Video