Presentation RD Cavin Kare Research Centre Cavin Kare

Presentation - R&D Cavin. Kare Research Centre Cavin. Kare Pvt. Ltd. Confidential Document : Access to Approved Users Only

Objectives of R&D • To contribute to the business objectives of CKPL by developing innovative, cost effective and superior products and processes • To make R&D a self-supporting / profit making activity by creating and developing Intellectual Property through out-licensing or national / international collaborations Confidential Document : Access to Approved Users Only

Product Portfolio Personal Care : Hair, Skin and Home Shampoo Hair Wash Powder Hair Dye Hair Oil Cream / Lotion Talc, Deo & Dab-on Toilet Bowl Cleaner Mosquito Coil Chik, Nyle, Meera, Karthika Indica Meera, Nyle Fairever, FE Mantra, Nyle CC & ML Spinz Tex Dishoom Foods : Ready-to-eat Pickles Spices Vermicelli Chinni’s, Ruchi Chinni’s Confidential Document : Access to Approved Users Only Sweets Ruchi Dry Fruits Snack Bar Chinni’s Moments Chinni’s Cruncho

R&D - Relationships Personal Care (Domestic Business) Foods (Domestic Business) International Business Salon Business (Trends in Vogue) Confidential Document : Access to Approved Users Only

R&D Strategy for Competitive Advantage To Provide Superior Products at Affordable Price Points • Superior Formulation (sensory experience; delivery of benefit; care) • Discovery of new ingredients (exclusivity; superior performance) • Developing new / improved processes (cost effectiveness) • Developing new technology (exclusivity; superior performance) • Strengthening biology (developing capability; studying new pathways) • Leveraging IPR (drafting claims; analysis; competitive intelligence) Confidential Document : Access to Approved Users Only

R&D Set-up & Facilities Confidential Document : Access to Approved Users Only

CRC at a Glance Buildings = 2 Total Build-up Area = 30000 Ft 2 Annual Expenditure Personnel Strength = @1% of Turnover = 40 (Projected 2007) (Technical) Educational Status Personnel Status (As on Aug 07) Doctorates 8 Masters 24 Graduates 4 Total Confidential Document : Access to Approved Users Only Number 36

Major Equipments (Analytical) • • Gas Chromatograph HPLC TLC Scanner UV Spectrophotometers Moisture analyser (Infrared, Karl-Fischer) Viscometer Mexameter Skin Testing Equipment (sebum level, moisture, p. H, elasticity, TEWL) • Hair Testing Equipment (tensile prop. , combability, volume) • Reflection Spectrophotometer Confidential Document : Access to Approved Users Only

Major Equipments (Infrastructural) • • • Stability chambers Rotary evaporator Clean air workbench Cold room Ultra-low Temp Fridge (-70°C) Washing Machine Soxhlet extractor Autoclave Tray Driers • • • Cell culture facility Tergetometer Triple Roll Mill Contra-Rotary Mixer Sigma Mixer Simplex Plodder Ribbon Mixer Horizontal Mixer Mini Soap Plodder Rotary coating pan • Generator Backup • Effluent Treatment Plant Confidential Document : Access to Approved Users Only

Information / Knowledge Management Information Systems • A fully networked facility with a central control (server) based on ‘thin client’ technology and a Document Management System • Sample Tracking for all analytical operations including work flow Confidential Document : Access to Approved Users Only

R&D Programme Confidential Document : Access to Approved Users Only

R&D Programme : Basic Research Extraction, isolation and characterisation of bioactive / functional compounds from natural sources Synthesis, characterisation and new process development of ingredients for personal care products Screening of natural products for microbiocidal activities with an emphasis on preservation of products and functional delivery (e. g. antidandruff) Characterisation of major ingredients presently used in personal care products to optimise their performance Confidential Document : Access to Approved Users Only

R&D Programme : Product Development of Formulations and Processes for Safe & Effective Products with emphasis on functional delivery in the areas of - Ø Hair Care Products Ø Skin Care Products Ø Cleaning and Hygiene Products Continuous optimisation and development of new formulation to keep the product cost under control Development of Spices, Vermicelli and Ready-to-Eat Products Such as Pickles, Nut based products (like peanuts, cashew), Snack foods and Traditional Sweets Confidential Document : Access to Approved Users Only

Outline of R&D Activities Confidential Document : Access to Approved Users Only

Product Development (Selection of Ingredients) • • Focus on Benefits Delivery of Promise (Claims substantiation) Superior Sensory Experience Gentle / Safe Confidential Document : Access to Approved Users Only

Product Development : Shampoo Typical Ingredients Typical Analysis Confidential Document : Access to Approved Users Only

Sensory Evaluation for Products Confidential Document : Access to Approved Users Only

Product Support • Identifying / Developing New Ingredients (biochemistry, microbiology & chemistry) • Developing Specifications & Methods (Measurement) • Developing New Processes (Chemistry) • Demonstrating Efficacy (Biochemistry, Claim substantiation) • Facing the Challenge of Contamination (Microbiology) • Information / Knowledge Management (Library) • Facilitating Activities (IT services, administration etc) Confidential Document : Access to Approved Users Only

Claim Substantiation • • Safety evaluation of Products e. g. Irritancy test Developing study protocol Identifying clinical investigator Preparing documents like Consent Form, Investigator Declaration Form, Product Brief, Assessment Form etc • Recruitment of volunteers • Coordination and conduct of the study • Data collation, Analysis and Report submission Confidential Document : Access to Approved Users Only

Hair Oil - Study Highlights (Claim Substantiation) Note : No. of hairs obtained is the number of hair collected during combing in the clinic Confidential Document : Access to Approved Users Only

Evaluating a Skin Lightening Cream (Claim Substantiation) Assessing Reduction in Pigmentation Volunteer Perception of Efficacy Reduction in Average Mexameter Reading % Reduction = 248 = 13. 95 % Confidential Document : Access to Approved Users Only

Biochemical Assays Mildness Assay (Shampoo)1. Zein assay 2. Collagen swelling 3. p. H rise Skin Lightening Assay 1. Tyrosinase inhibition assay 2. DOPA autooxidation inhibition assay Anti-aging Assay Elastase inhibition asssay Antiinflammatory Assay Soyabean lipooxygenase inhibition assay Antioxidant Assay 1. DPPH radical scavenging assay 2. Deoxyribose degradation assay 3. Superoxide Dismutase assay Confidential Document : Access to Approved Users Only

Cell Based Assays Skin Lightening 1. Melanin assay 2. Tyrosinase inhibition assay Oxidative stress Cytotoxicity Assay Cytotoxicity / Proliferation MTT assay Mildness Assay (Shampoo) 1. Cytotoxicity assay 2. RBC lysis assay Cell lines Available : Melanocyte (melanoma) Fibroblast (normal) Keratinocyte (carcinoma) General (cancer) Primary cell lines Confidential Document : Access to Approved Users Only B 16 -F 10, B 16 -F 1 & G 361 NIH / 3 T 3 A 431 He. La Fibroblasts & Keratinocytes

Chemistry Extraction, isolation and characterisation of bioactive / functional Compounds from natural sources (skin lightening, antioxidants, antiaging etc. ) Synthesis, characterisation and new process development of Ingredients for personal care products Screening of natural products for microbiocidal activities with an Emphasis on preservation of products and functional delivery (e. g. antidandruff) Development of natural hair colours Confidential Document : Access to Approved Users Only

Chemical Process Oleochemicals Secondary Surfactants 1. Monoethanol amides 2. Amidopropyl betaines Also useful as Thickening Agent Confidential Document : Access to Approved Users Only

Microbiology • • • Study of anti-microbial efficacy (MIC, synergy etc) Selection of suitable preservatives for products Estimation of microbial load during development Preservative Challenge Test Screening natural products for antidandruff activity Evaluation of competitive products Confidential Document : Access to Approved Users Only

Measurements Total No. Assays = >190 Major Responsibilities • Analysis of Own Products • Analysis of Competitor Products • Setting up Specifications • Interfacing with Regulatory Authorities (BIS, Drug Controllers) Sample Tracking Confidential Document : Access to Approved Users Only

Confidential. Document: : Accesstoto. Approved. Users. Only