Prepare a Wet Mount CAPT Mike Hughey MC

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Prepare a Wet Mount CAPT Mike Hughey, MC, USNR Operational Obstetrics & Gynecology ·

Prepare a Wet Mount CAPT Mike Hughey, MC, USNR Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide

First, a Video Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery ·

First, a Video Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 2

Obtain a Specimen • Spatula • Cotton-tipped Applicator • Obtain directly or from inside

Obtain a Specimen • Spatula • Cotton-tipped Applicator • Obtain directly or from inside curve of speculum Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 3

Place on Microscope Slide • Use very small amount • Too much makes the

Place on Microscope Slide • Use very small amount • Too much makes the slide too thick and unreadable Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 4

Add Na. Cl and KOH • One drop • Mix well • Two slides

Add Na. Cl and KOH • One drop • Mix well • Two slides vs. one slide? Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 5

Add Coverslip • Remove excess fluid so the coverslip is stable. • Heat the

Add Coverslip • Remove excess fluid so the coverslip is stable. • Heat the KOH slide? Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 6

Microscopic Examination • Start with low power • Rarely need to go above medium

Microscopic Examination • Start with low power • Rarely need to go above medium power • Focus up and down through the plane of the cells Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 7

Yeast Tangle of threads Single branch Operational Obstetrics & Gynecology · Bureau of Medicine

Yeast Tangle of threads Single branch Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 8

Watch this video Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery ·

Watch this video Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 9

Trichomonas • About the same size as a white blood cell • Smaller than

Trichomonas • About the same size as a white blood cell • Smaller than a vaginal epithelial cell • Violent motion Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 10

Watch this video Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery ·

Watch this video Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 11

Bacterial Vaginosis Clue Cell • Clue cells • Vaginal epithelial cells studded with bacteria

Bacterial Vaginosis Clue Cell • Clue cells • Vaginal epithelial cells studded with bacteria • Smudged, granular appearance • Indistinct borders Normal Vaginal Epithelial Cell Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 12

Clue Cells Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000

Clue Cells Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 13

Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 14

Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 14