Prepare a Wet Mount CAPT Mike Hughey MC
- Slides: 14
Prepare a Wet Mount CAPT Mike Hughey, MC, USNR Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide
First, a Video Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 2
Obtain a Specimen • Spatula • Cotton-tipped Applicator • Obtain directly or from inside curve of speculum Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 3
Place on Microscope Slide • Use very small amount • Too much makes the slide too thick and unreadable Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 4
Add Na. Cl and KOH • One drop • Mix well • Two slides vs. one slide? Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 5
Add Coverslip • Remove excess fluid so the coverslip is stable. • Heat the KOH slide? Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 6
Microscopic Examination • Start with low power • Rarely need to go above medium power • Focus up and down through the plane of the cells Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 7
Yeast Tangle of threads Single branch Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 8
Watch this video Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 9
Trichomonas • About the same size as a white blood cell • Smaller than a vaginal epithelial cell • Violent motion Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 10
Watch this video Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 11
Bacterial Vaginosis Clue Cell • Clue cells • Vaginal epithelial cells studded with bacteria • Smudged, granular appearance • Indistinct borders Normal Vaginal Epithelial Cell Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 12
Clue Cells Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 13
Operational Obstetrics & Gynecology · Bureau of Medicine and Surgery · 2000 Slide 14