Practical Microbiology Ibrahim Mahmoud Sayed Ibrahim Instruments 1
Practical Microbiology Ibrahim Mahmoud Sayed Ibrahim
Instruments 1 -Seitz filter 2—Mac. Knotoch 3 -Gas Pack system
2 - IMVC test IMVC of E. coli • •
IMVC test Klebsellia
TSI (triple sugar iron) LF (Ecoli) Pseudomonas Proteus •
Christen urea media Pink +ve proteus Yellow –ve Klebellia Simmon Citrate Green –ve Ecoli • Blue + ve •
Sugar fermentation of Salmonella S. paratyphi B • • S. typhi
Sugar fermentation of Shigella ysentriae Sh. flexenerii Sh. Sonneii •
Sugar Fermentation of Neisseria • N. meningitidis
Non-inoculated Media Loffler’s serum media Dorset Egg Lowstein Jensen •
Non inoculated media Anaerobic media Thioglycolate –Robertson cooked meat-Deep agar
II inoculated media Nutrient Agar Staph (endopigment) (Exopigment) Proteus (swarming) Pseudomonas Sabroud dextrose (yeast)
II-Blood agar (B-hemolysis) as Staph aureus, Strept Pyogens • Blood tellurite as Corynebacterium diphtheriae •
III Chocholate agar Pneumococci Optochin sensitivity • + ve zone : Pneumococci • - ve zone : S. viridans •
IV) Pink Plate Mannitol salt agar Staph aureus Mac. Conkey Agar • Lf ( Ecoli) •
V) EMB plate E. coli Klebsellia •
VI) Special Plate Mac. Conkey Agar NLF(Salmonella) Sabroud dextrose Agar • Mould •
VI) Special Plate Milk Agar Staph Bismuth Sulphite • Salmonella •
- Slides: 18