POWERPOINT LECTURE SLIDE PRESENTATION by LYNN CIALDELLA MBA
POWERPOINT® LECTURE SLIDE PRESENTATION by LYNN CIALDELLA, MBA, The University of Texas at Austin UNIT 1 5 PART B Membrane Dynamics HUMAN PHYSIOLOGY AN INTEGRATED APPROACH DEE UNGLAUB SILVERTHORN Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings FOURTH EDITION
Carrier-Mediated Transport § Specificity § Competition § Saturation § Transport maximum Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings
Vesicular Transport § Phagocytosis § Cell engulfs bacterium or other particle into phagosome § Endocytosis § Membrane surface indents and forms vesicles § Active process which can be nonselective (pinocytosis) or highly selective § Potocytosis uses caveolae § Receptor-mediated uses clathrin-coated pits Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings
Receptor-Mediated Endocytosis and Exocytosis 1 Ligand binds to membrane receptor. 9 Exocytosis Extracellular fluid 2 Receptor-ligand migrates to clathrin-coated pit. 8 Transport vesicle and cell membrane fuse (membrane recycling). Clathrincoated pit 3 Endocytosis Receptor Clathrin 7 Transport vesicle with receptors moves to the cell membrane. 5 Receptors and ligands separate. To lysosome or Golgi complex 6 Ligands go to lysosomes or Golgi for processing. Endosome Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings 44 Vesicle loses clathrin coat. Intracellular fluid Figure 5 -24
Receptor-Mediated Endocytosis and Exocytosis 1 Ligand binds to membrane receptor. Extracellular fluid Receptor Intracellular fluid Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -24, step 1
Receptor-Mediated Endocytosis and Exocytosis 1 Ligand binds to membrane receptor. Extracellular fluid 2 Receptor-ligand migrates to clathrin-coated pit. Clathrincoated pit Receptor Clathrin Intracellular fluid Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -24, steps 1– 2
Receptor-Mediated Endocytosis and Exocytosis 1 Ligand binds to membrane receptor. Extracellular fluid 2 Receptor-ligand migrates to clathrin-coated pit. Clathrincoated pit 3 Endocytosis Receptor Clathrin Intracellular fluid Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -24, steps 1– 3
Receptor-Mediated Endocytosis and Exocytosis 1 Ligand binds to membrane receptor. Extracellular fluid 2 Receptor-ligand migrates to clathrin-coated pit. Clathrincoated pit 3 Endocytosis Receptor Clathrin 44 Vesicle loses clathrin coat. Intracellular fluid Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -24, steps 1– 4
Receptor-Mediated Endocytosis and Exocytosis 1 Ligand binds to membrane receptor. Extracellular fluid 2 Receptor-ligand migrates to clathrin-coated pit. Clathrincoated pit 3 Endocytosis Receptor Clathrin 5 Receptors and ligands separate. Endosome Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings 44 Vesicle loses clathrin coat. Intracellular fluid Figure 5 -24, steps 1– 5
Receptor-Mediated Endocytosis and Exocytosis 1 Ligand binds to membrane receptor. Extracellular fluid 2 Receptor-ligand migrates to clathrin-coated pit. Clathrincoated pit 3 Endocytosis Receptor Clathrin 5 Receptors and ligands separate. To lysosome or Golgi complex 6 Ligands go to lysosomes or Golgi for processing. Endosome Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings 44 Vesicle loses clathrin coat. Intracellular fluid Figure 5 -24, steps 1– 6
Receptor-Mediated Endocytosis and Exocytosis 1 Ligand binds to membrane receptor. Extracellular fluid 2 Receptor-ligand migrates to clathrin-coated pit. Clathrincoated pit 3 Endocytosis Receptor Clathrin 7 Transport vesicle with receptors moves to the cell membrane. 5 Receptors and ligands separate. To lysosome or Golgi complex 6 Ligands go to lysosomes or Golgi for processing. Endosome Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings 44 Vesicle loses clathrin coat. Intracellular fluid Figure 5 -24, steps 1– 7
Receptor-Mediated Endocytosis and Exocytosis 1 Ligand binds to membrane receptor. Extracellular fluid 2 Receptor-ligand migrates to clathrin-coated pit. 8 Transport vesicle and cell membrane fuse (membrane recycling). Clathrincoated pit 3 Endocytosis Receptor Clathrin 7 Transport vesicle with receptors moves to the cell membrane. 5 Receptors and ligands separate. To lysosome or Golgi complex 6 Ligands go to lysosomes or Golgi for processing. Endosome Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings 44 Vesicle loses clathrin coat. Intracellular fluid Figure 5 -24, steps 1– 8
Receptor-Mediated Endocytosis and Exocytosis 1 Ligand binds to membrane receptor. 9 Exocytosis Extracellular fluid 2 Receptor-ligand migrates to clathrin-coated pit. 8 Transport vesicle and cell membrane fuse (membrane recycling). Clathrincoated pit 3 Endocytosis Receptor Clathrin 7 Transport vesicle with receptors moves to the cell membrane. 5 Receptors and ligands separate. To lysosome or Golgi complex 6 Ligands go to lysosomes or Golgi for processing. Endosome 44 Vesicle loses clathrin coat. Intracellular fluid Exocytosis is the opposite of endocytosis Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -24, steps 1– 9
Transepithelial Transport Polarized cells of transporting epithelia Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -25
Transepithelial Transport of Glucose [Glucose] low Lumen of kidney or intestine Glu Na+ [Na+] high 1 1 Na+ glucose symporter brings glucose into cell against its gradient using energy stored in the Na+ concentration gradient. 2 GLUT transporter transfers glucose to ECF by facilitated diffusion. Apical membrane [Glucose] high Glu Na+ [Na+] low Epithelial cell Basolateral membrane Glu Na+ 3 Na+ -K+- ATPase pumps Na+ out of the cell, keeping ICF Na+ concentration low. K+ 2 3 ATP Extracellular fluid [Glucose] low Glu [Na+] high Na+ K+ Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -26
Transepithelial Transport of Glucose Lumen of kidney or intestine [Glucose] low Glu Na+ [Na+] high 1 1 Na+ glucose symporter brings glucose into cell against its gradient using energy stored in the Na+ concentration gradient. Apical membrane [Glucose] high Glu Na+ [Na+] low Epithelial cell Basolateral membrane Extracellular fluid Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -26, step 1
Transepithelial Transport of Glucose [Glucose] low Lumen of kidney or intestine Glu Na+ [Na+] high 1 1 Na+ glucose symporter brings glucose into cell against its gradient using energy stored in the Na+ concentration gradient. 2 GLUT transporter transfers glucose to ECF by facilitated diffusion. Apical membrane [Glucose] high Glu Na+ [Na+] low Epithelial cell Basolateral membrane Glu 2 Extracellular fluid [Glucose] low Glu Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -26, steps 1– 2
Transepithelial Transport of Glucose [Glucose] low Lumen of kidney or intestine Glu Na+ [Na+] high 1 1 Na+ glucose symporter brings glucose into cell against its gradient using energy stored in the Na+ concentration gradient. 2 GLUT transporter transfers glucose to ECF by facilitated diffusion. Apical membrane [Glucose] high Glu Na+ [Na+] low Epithelial cell Basolateral membrane Glu Na+ 3 Na+ -K+- ATPase pumps Na+ out of the cell, keeping ICF Na+ concentration low. K+ 2 3 ATP Extracellular fluid [Glucose] low Glu [Na+] high Na+ K+ Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -26, steps 1– 3
Transcytosis across the Capillary Endothelium Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -27
Transcytosis across the Capillary Endothelium Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -27 (1 of 3)
Transcytosis across the Capillary Endothelium Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -27 (2 of 3)
Transcytosis across the Capillary Endothelium Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -27 (3 of 3)
Transcytosis across the Capillary Endothelium Copyright © 2007 Pearson Education, Inc. , publishing as Benjamin Cummings Figure 5 -27
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