Posidonia oceanica a usefull tool to biomonitor the
Posidonia oceanica, a usefull tool to biomonitor the pollution of Mediterranean coastal areas by trace elements J. Richir, R. Biondo, J. -M. Bouquegneau, S. Gobert. Bruxelles 02 -12 -11
Introduction Trace elements I N T R O D U C T I O N Ecotoxicology pollution bioindicators = organisms accumulating pollutants to levels representative of their habitat pollution status. P. oceanica M. galloprovincialis
Introduction Trace elements I N T R O D U C T I O N Ecotoxicology pollution bioindicators = organisms accumulating pollutants to levels representative of their habitat pollution status. P. oceanica M. galloprovincialis
Introduction Trace elements I N T R O D U C T I O N biomonitoring Ecotoxicology TE Cd Pb Cu Zn Cr Fe Ni # ref. 34 29 27 25 22 14 13 TE As Se Ag Co Mn Al # ref. 3 3 2 2 2 1 TE Be Bi V Mo Sn Sb # ref. 0 0 0 Objectives: v [TEs] in Posidonia oceanica - baselines v spatial variation - contamination sources v kinetics - accumulation vs. excretion v tissue speciation - preferential accumulation routes v plant physiology / seasonality vs. [TEs]
Stations PACA-Corsican coasts E X A M P L E 1
Bi - 18 stations PACA-Corsican coasts E X A M P L E 1
Bi - Ajaccio E X A M P L E 2 H 9 - H 1
Bi - Ajaccio E X A M P R L E E S U 2 L T S H 9 H 8 H 7 H 6 H 9 - H 5 H 1 H 4 H 3 H 2 H 1
Bi - Ajaccio E X A M P L E 2 H 9 H 8 H 7 H 6 H 5 H 4 H 3 H 2 H 1
In situ TE contamination E X A M P L E 3 • 6 days of contamination; • 15 TEs (Pb, Co, Ag, Al, Mn, etc. ); • 410 L bell-shaped mesocosm; • Contamination every 12 hours (9 am -9 pm); • 15 days of decontamination.
In situ TE contamination E X A M P L E 3
Pb kinetics E X A M P L E 3
Tissue compartmentalization blade orthotropic shoot plagiotropic shoot B C limb rhizome roots D E leaf base (petiole) ligula leaf base stipule scale rhizome root (A) shoot of leaves on a plagiotropic rhizome; (B, C) adult leaves; (D) intermediate leaf ; (E) juvenile leaf (Libes and Boudouresque, 1987). A
Tissue compartmentalization - leaves E X A M P L E 1. V 2. Zn Hypotheses: Ø water column accumulation Ø residence time Ø dilution effect 4 IL 18, 7 ± 5, 8 157 ± 7 BAL SAL
Above- vs. bellow-ground tissues E X A M P L E 5
Plant physiology - shoot weight vs. V E X A M P L E 6
Plant physiology - shoot weight vs. V [TEs] in Posidonia oceanica C O N C L U S I O N S v spatial variation - contamination sources - baselines e. g. Bi : PACA-Corsican coasts and Ajaccio Bay v kinetics - accumulation vs. excretion e. g. Pb v tissue speciation - preferential accumulation routes e. g. V, Zn v plant physiology / seasonality vs. [TEs] e. g. V vs. shoot weight
Q U E S T I O N S Thank you for your attention
Introduction fluxes; Geophysicochemistry I N T R O D U C T I O N spatial distribution; speciation, balances, … Trace elements Ecology ecosystems; organisms. Ecotoxicology adverse effects; pollution, … newsvine. com corsematin. com flickr. com
Laboratory analyses M A T E R I A L Analytical steps: adult leaves shoots 1/6/9 Calvi Ethos D • acidic digestion in a microwave oven • measures : Ø inductively coupled plasma mass spectrometer M E T H O D S • homogeneous sample ELAN DRCII
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