PLASMA MEMBRANE Plasma Membrane Boundary that separates the
PLASMA MEMBRANE
Plasma Membrane • • Boundary that separates the living cell from it’s non-living surroundings. Phospholipid bilayer Amphipathic - having both: hydrophilic heads hydrophobic tails Phospholipid ~8 nm thick
Plasma Membrane - cont. • Controls traffic into and out of the cell with phospholipids and transport proteins. • Selectively permeable Transport protein
Selective Permeability • The property of biological membranes which allows some substances to cross more easily than others.
Fluid Mosaic • 1972 - Singer and Nicolson called the membrane a “Fluid Mosaic Model”. • Mosaic: different proteins embedded in the phospholipids. • Fluid: proteins and phospholipids can move freely in the membrane.
Fluid Mosaic - cont. • Components of a phospholipid bilayer. 1. 2. 3. 4. 5. 6. phospholipids proteins - enzymes, receptors, transport. glycolipids glycoproteins carbohydrates cholesterol
Transport Proteins • Transports molecules or ions across biological membranes • 3 types of transport proteins: proteins 1. uniport 2. symport 3. antiport
Uniport Transport Protein • Carries a single solute across the membrane. extracellular fluid intracellular fluid
Symport Transport Protein • Translocate 2 different solutes simultaneously in same direction. extracellular fluid intracellular fluid
Antiport Transport Protein • Exchanges 2 solutes by transporting them in opposite directions extracellular fluid intracellular fluid
Diffusion • The net movement of a substance (molecules) down a concentration gradient from an area of high concentration to an area of low concentration • passive transport: NO energy is expended. • facilitated diffusion: type of passive transport which uses transport proteins.
Osmosis • The movement of water across selectively permeable membranes • The water moves from a high concentration to low concentration
Question: What’s in a Solution? Answer: • solute + solvent • Na. Cl H 20 + solution saltwater
Hypertonic • A solution with a greater solute concentration compared to another solution 3% Na. Cl 97% H 2 O Red Blood Cell solution 5% Na. Cl 95% H 2 O
Hypotonic • A solution with a lower solute concentration compared to another solution 3% Na 97% H 2 O Red Blood Cell solution 1% Na 99% H 2 O
Isotonic • A solution with an equal solute concentration compared to another solution 3% Na 97% H 2 O Red Blood Cell solution 3% Na 97% H 2 O
Movement of H 2 O • Water will “ALWAYS” diffuses down a concentration gradient from a HYPOTONIC solution to a HYPERTONIC solution “ALWAYS REMEMBER” • HYPOTONIC HYPERTONIC
Animal Cells • Animal cells placed into a hypotonic solution will HEMOLYSIS (EXPLODE). • Animal cells placed into a hypertonic solution will CRENATE (SHRIVEL). Hemolysis Crenation Red Blood Cells
Plant Cells • Firmness or tension (vacuole full) that is found in plant cells (cell wall) that are in a hypotonic environment is called TURGID. • This process is called TURGOR PRESSURE. Water Cell Water Central Vacuole Water
Plant Cells • When the plasma membrane pulls away from the cell wall (vacuole empty) in a hypertonic environment (loss of water) is called PLASMOLYSIS Water plasma membrane Cell Water
Active Transport • The movement of molecules (small or large) across the plasma membrane in which energy (ATP) is required • Examples: 1. 2. 3. Sodium (Na) - Potassium (K) Pump Exocytosis Endocytosis
Sodium-Potassium Pump • The mechanism that uses energy (active transport) released from splitting ATP to transport Sodium (Na+) out of and Potassium (K+) into cells. extracellular fluid intracellular fluid K+ K+ Na+
Question: • How are large molecules transported into and out of the plasma membranes?
Answer: • Exocytosis and Endocytosis
Exocytosis • Cell secretes macromolecules (proteins and other biochemicals) out of cell. • Part of the Endomembrane System: the fusion of transport vesicles with plasma membrane.
Endocytosis • The energy requiring movement of particles (foreign or natural) into the cell. • 3 types of endocytosis: A. Phagocytosis B. Pinocytosis C. Receptor-mediated endocytosis
A. Phagocytosis • Cell eating: cells engulf particles with pseudopodia and pinches off a food vacuole. Bacteria • Two examples: 1. White Blood Cell 2. Amoeba Food Vacuole White Blood Cell
B. Pinocytosis • Cell drinking: droplets of extracellular fluid are absorbed into the cell by small vesicles • Example: 1. Fungi Hyphae Food Particles
C. Receptor-Mediated Endocytosis • Importing specific macromolecules (hormones) into the cell by the inward budding of vesicles formed from coated pits (receptors). Liver Cell Hormones Receptors
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