Plant Propagation by Cuttings a Kinds of cuttings

Plant Propagation by Cuttings a. Kinds of cuttings 1) Stem cuttings 2) Leaf-bud cutting (single-eye) 3) Root cuttings 4) Leaf cuttings b. Totipotency-The ability of an individual cell to reconstitute the entire plant part and functions c. Dedifferentiation-The capability of previously developed, differentiated cell to return to a meristem

Plant Propagation by Cuttings 1. Stem cuttings 2. Leaf-bud cutting (singleeye) 3. Root cuttings 4. Leaf cuttings

1. Stem Cuttings • • Softwood Cuttings Semi-Hardwood Cuttings Herbaceous Cuttings

Softwood Cuttings

Shoftwood Cuttings - Hydrangea Butterfly cutting (top) Double-eye single node cutting (middle) Single-eye single node cuttings (bottom)

Softwood Cuttings-Peach - Actively growing shoots are used - Softwood cuttings are taken during spring and summer

Influence of IBA on Semi-Hardwood Cuttings - Cordia 1 -Control, 2 -50% ethanol, 3 -100 ppm, 4 -1000 ppm, 5 -2000 ppm, 6 -4000 ppm, 7 -6000 ppm, 8 -8000 ppm, 9 -10000 ppm IBA

Semi-Hardwood Cuttings - Jojoba Treating cuttings in IBA Solution (top) Sticking IBA-treated cuttings in root substrate (bottom)

Bottom Heat System for Cutting Benches

Temperature Differential Helpful for Root Formation Ambient Temp 60 o. F - Rooting requires carbohydrates (energy), auxin (growth hormone), and rooting cofactors (enzymes) - Temperature differential (10 o. F) between the ambient air and root zone is helpful for faster rooting Root zone Temp 70 o. F

Good sanitation is important Cutting on the left was infected with Alternaria and did not root Cutting on the right was healthy and rooted well

Cutting Propagation in Jojoba • Regular Stem Cuttings – Use 4 -5 nodes – Semi-hardwood cuttings • Single Node Cuttings – Double-eye single node cuttings – Single-eye single node cuttings

Rooting of 3 Different Types of Cuttings in Jojoba Regular Cuttings Single Node Cuttings Double Eye (DE) 5 -node Single Eye (SE)

Single Node Cuttings Double-Eye Single Node Cutting (DE) - Healthier than SE - Less disease attacks Single-Eye Single Node Cutting (SE) - Largest no. cuttings/plant - Slower development - Higher mortality