Plant Chaperones Lab Prof Adina Breiman 517 Britania

Plant Chaperones Lab חלבונים נלווים Prof. Adina Breiman פרופ' עדינה ברימן 517, Britania, Tel: 03 -6409377 (office), 03 -6409845 (lab) adina@post. tau. ac. il; http: //www. tau. ac. il/lifesci/plant_sciences/USR/breiman/ Our studies are focused on the study of the family of co chaperones with the enzymatic activity of peptidyl prolyl cis trans isomerases (PPIases) in plants. This large family possess several families the cyclophilins , FKBPs and parvulins. Members of these families were shown to play important roles in signal transduction and protein folding due to their activity as chaperones and PPIases. We chose to work on the FKBP family because members of this have important roles in signal transduction in mammals and yeast. The FKBP family is highly conserved among all organisms and after studying two members of this family in wheat , we have switched to the Arabidopsis system because of the big genetic potential of Arabidopsis mutants and genomics. We study members of the FKBP family which are heat shock proteins, bind the heat shock protein HSP 90 , calmodulin and dynein. By using mutants missing the FKBP genes Rof 1 and Rof 2 and by over expressing those genes we plan to understand their role in signal transduction and molecular chaperone activity. Recent Publications: Kurek, I. , R. Dulberger, D. Sudhakar, P. Christou & A. Breiman, 2002. Deletion of the C terminal 138 amino acids abrogates calmodulin binding, dimerisation and male fertility in transgenic rice. Plant Mol. Biol. 48, 369 -381. Kurek I. , F. Pirkl, E. Fischer, J. Buchner & A. Breiman, 2002. The wheat FKBP 73 functions in vitro as a molecular chaperone independent of its peptidyl prolyl cis -trans isomerase activity. Planta 215, 119 -126. Kurek. I , E. Stoger, R. Dulberger, P. Christou & A. Breiman 2002. Over expression of the wheat FKBP 77 and the heat induced FKBP 77 in the transgenic wheat reveals different function of the two isoforms Transgenic Research 11, 373 -379. Graduate Students Samuel Bocovsa Ruth Wilunsky Yulia Skovorodnikov