PILATUS A 2 Dimensional XRay Detector For Macromolecular

PILATUS: A 2 -Dimensional X-Ray Detector For Macromolecular Crystallography E. F. Eikenberry 1 Ch. Brönnimann 1 G. Huelsen 1 H. Toyokawa 2 R. Horisberger 3 B. Schmitt 1 C. Schulze-Briese 1 T. Tomizaki 1 Swiss Light Source, Paul Scherrer Institut, CH-5232 Villigen-PSI SPring 8, Japan 3 CMS-Project, Paul Scherrer Institut, CH-5232 Villigen-PSI 1 2 E. F. Eikenberry

Su Int rface Mi erfac s / cro es sc : op y SLS Layout 1 L 12 S 11 M IR VUV 2 S 3 M LINAC (100 Me. V) 10 S 4 S Storage Ring (2. 4 Ge. V, 288 m) Undulators 9 L 5 L 8 S S 7 M XA 10 m in e t Pro llo. Xta phy gra 6 S Materials Sciences s/ ce : y rfa es Su erfac scop Int ectro Sp Booster (2. 4 Ge. V) Green: first phase beamlines Red: proposed beamlines E. F. Eikenberry

Paul Scherrer Institut Data collection in protein crystallography SLS Detector Group

X-Ray Diffraction From A X-174 Virus Crystal SLS Detector Group

Macromolecular Crystallography Determine the structure of highly complex molecules to atomic resolution Molecular Weight > 1 MDa (> 50, 000 atoms) Applications: Enzymes Viruses Catalytic mechanisms Electron transport Energy transduction Motility Drug Design Structural genomics SLS Detector Group

Paul Scherrer Institut The SLS Pixel Detector & Size: 40 x 40 cm 2 (0. 16 m 2) & 2000 x 2000 pixels & Pixel size: 217 x 217 m 2 & Modular detector -> dead area ~6% & High frame rate: >10 Hz & High duty cycle: >94% (Tro~6 ms) Base plate (water cooled) Bank with 5 modules Tilt angle: variable Modules with 16 chips SLS Detector Group

Paul Scherrer Institut Pixel Detectors: Principle Si pn-junction 3. 6 e. V to create 1 eh-pair Detector Radiation hard Pixel electronics Treshold correction Bump Pad Global Tresh CS Amp 1. 7 f. F Cal Comp + Enable/ Disable Analog Block Ext/Comp Clock 12 Clock Gen Ext Clock 15 bit SR counter RBI RBO Reset Digital Block SLS Detector Group

Please see landscape-oriented supplement: Slide 8: Bump-bonded Module Slide 9: PILATUS Module Slide 10: Final Module Assembly Slide 11: Bank With 3 Modules Slide 12: Module Fabrication Slide 15: Flat-field Correction Slide 18: Continuous Fine -Slicing Slide 19: Lysozyme: MOSFLM Autoindex Slide 20: Lysozyme: MOSFLM Refined Cell

Trimming A Module With A Global Vcmp SLS Detector Group

Threshold vs. Vcmp SLS Detector Group

PILATUS Detector Crystallography Lysozyme 1º rotation 2 s exposure SLS Detector Group

Fine -Slicing with PILATUS 1. 5° Rotation Image Integrated Intensity, x-rays Lysozyme: 0. 02° steps 0. 5 s exposure FWHM: 0. 06° - 0. 10° Rotation Angle ( ), deg. SLS Detector Group

SLS 2 k Pixel Detector PIXEL: CHIP: 217 m 2 Preamplifier and shaper Threshold discriminator with 4 -bit trim 15 -bit counter >105 x-ray/s CMOS 0. 8 m DMILL rad-hard Designed at PSI 44 78 pixels, 9. 9 18. 3 mm 2 Read out at 10 MHz MODULE: Fully depleted diode array 81 36. 6 mm 2 300 m, 57, 462 pixels Indium bump-bonded to 16 chips Chips read out in parallel (5 ms) Module control board BANK: 5 modules Bank control board caches data DETECTOR: 12 banks Adjustable V-shape With fine-slicing, a 180 rotation may produce 2000 8 MB images (16 GB) in ~3 min. SLS Detector Group