Phyla 1 Basidiomycota 2 Ascomycota 3 Zygomycota 4

真菌界 現有五個門 (Phyla) 1. 擔子菌門 (Basidiomycota) 2. 子囊菌門 (Ascomycota) 3. 結合菌門 (Zygomycota) 4. 壺菌門 (Chytridiomycota) 5. 聚合菌門 (Glomeromycota)

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菌種 枯木上的 Halorosellinia oceanica 、 Nemania maritima 、 Patellaria atrata －海洋 子囊菌 海洋酵母菌 -海洋子囊菌 海洋無性真菌 Dendryphiella sp. -海洋子囊菌

CELLULOSE � � A long and linear homopolymer consisting of -D-glucose connected by (1 4) glycosidic linkages Cellulose is the most abundant organic polymer in this biosphere Associates with lignin, hemicelluloses and pectic substances to form wood Only certain fungi and bacteria are capable of degrading wood

HEMICELLULOSE A group of short chain and less crystalline polysaccharides including glucose, xylose, mannose, galactose, arabinose, rhamnose and uronic acids Major portion and most abundant and renewable hemicellulose is xylan which is the polymer of xylose

WOOD-DEGRADING ENZYMES Cellulase hydrolysis of cellulose Xylanase degrade the linear polysaccharide beta-1, 4 -xylan into xylose

COMMERCIAL USES Cellulase textile industry laundry detergents pulp and paper industry Xylanase chlorine-free bleaching of wood pulp

實驗改動 第一、二組：Halophytophthora sp. 146 第三、四組：Halophytophthora sp. 147 第五、六組：Halophytophthora sp. 149 第七、八組：Halophytophthora sp. 159 第九、十組：Halophytophthora sp. 161

實驗流程 BX CMC Inoculate duplicate plates BX

染色 CMC plates ONLY 0. 2 mg/ml Congo red for 30 min 1 1 M Na. Cl for 5 min 2 5% acetic acid for 5 min 1

漆氧化酵素活性計算公式 漆氧化酵素活性(m. U/ml) = (吸光值增加量 /ABTS extinction coefficient in 96 well 200 l loading) 稀釋倍率 = (吸光值增加量/20. 608 103 ml/nmol) 100 *loading 200 l in NUNC 96 well plate *ABTS extinction coefficient at 405 nm=36. 8 m. M -1 cm-1 0. 56