Photosynthesis Energy Transformations ATP is the energy molecule

Photosynthesis

Energy Transformations ATP § is the energy molecule used in all living things, so supplies must be constantly replenished. § energy must be added to make ATP, thus ATP formation is an endergonic reaction. § ADP + energy --- ATP § the addition of a phosphate molecule to ATP is called phosphorylation § ATP --- ADP + energy § the removal of a phosphate molecule from ATP gives off energy and is called dephosphorylation

Electron Transport System (ETS) § is the means by which energy production occurs within cells. It is a series of progressively stronger electron acceptors. Each time an electron is transported, energy is either released or absorbed. § This is how most of the ATP in plants and animals is formed

Redox Reactions § Oxidation – loss of electrons, producing a substance with a more positive charge. § Reduction – gain of electrons, producing a substance with a more negative ( or less positive) charge.

Photosynthesis § Photosynthesis is the most important chemical process on earth. Photosynthesis harnesses sunlight energy and converts it into a form of energy (glucose and starch) that is useable by all living organisms. The process also produces oxygen, which is essential for higher life forms. § CO 2(g) + H 2 O(l) + light C 6 H 12 O 6(s) + O 2(g) + H 2 O(l) Sunlight energy is converted into chemical potential energy (glucose)

Site of Photosynthesis occurs within the chloroplasts of plant cells, within the thylakoid membrane and the stroma.

Chloroplasts - contain the photosensitive pigment chlorophyll, which reflects green light. Caroteniods – other accessory pigments that absorb blue and violet light, but reflect yellow. Stroma – interior space of the Chloroplast, The dark reaction occurs here. Thylakoid disk – photosynthetic membrane, where the light reactionoccurs. Grana – stacks of thylakoid disks. Lamellae – unstacked thylakiods between grana.

Stages of Photosynthesis Light Reactions – Thylakoid Membrane Stage 1: Solar energy is captured and transferred to the electrons, and water is split. § Photolysis – the splitting of water with light. § 2 H 2 O(l) + light --- 4 H+(aq) + 4 e- + O 2(g) § http: //www. stolaf. edu/people/giannini/flashanima t/metabolism/photosynthesis. swf

Photosystems § Within the chloroplasts, chlorophyll is found in clusters within the thylakoid membranes. These clusters are called photosystems. When light hits the photosystem, energy is absorbed and electrons are promoted to an electron acceptor at a higher energy level. As the electrons fall down the electron transport chain, energy is released and can be used to make ATP. Net. Learning - Examples – Flash Photosynthetic Electron Transport and ATP Synthesis

Stage 2: Electron transfer and the production of ATP. § Light energy is captured by the pigments within the photosystems and is used to form more stable energy rich molecules, and to make ATP from ATP and phosphate. This occurs by cyclic photophosphorylation and chemiosmosis.

§ Chemiosmosis – As H+ ions are pulled across the thylakoid membrane by the ETC, an concentration gradient of H+ ions is created. H+ ions travel down the concentration gradient, across the membrane, through an ATP synthase complex. As they do this, ATP is produced. § http: //student. ccbcmd. edu/~gkaiser/biotutorials/p hotosyn/hpumpp. html § Animation of Chemiosmosis Proton Pumping

Dark Reactions – Stroma Stage 3: Calvin Benson cycle and carbon fixation. § The Calvin Benson cycle uses ATP, high energy electrons, NADPH and CO 2 to produce glucose in the stroma. Sunlight energy is not required. § Ru. BP, a 5 carbon sugar acts as a CO 2 acceptor § The resulting 6 carbon sugar is split into two 3 carbon PGA molecules. § Using H from NADPH, PGA is converted to PGAL § PGAL can be used to produce glucose, starch, sucrose, glycerol or cellulose. § PGA can also be converted into amino acids or fatty acids.

Calvin-Benson Cycle The Calvin Benson cycle must turn 3 times to produce each PGA molecule, and 6 times to produce one glucose molecule. The plant cell can manufacture all necessary organic compounds from the products of photosynthesis. 6 CO 2(g) + 12 H 2 O(l) C 6 H 12 O 6(s) + 6 O 2(g) + 6 H 2 O(g) Calvin cycle

Photosynthesis Animated… § Photosynthesis § photosynthesis dark phase